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Pregled bibliografske jedinice broj: 1024064

Qualitative and quantitative NMR in optimization of methyl β-D-fructofuranoside synthesis catalyzed by invertase glycoforms


Andjelković, Uroš; Gudelj, Ivan; Klarić, Thomas; Hinneburg, Hannes; Vinković, Marijana; Wittine, Karlo; Dovezenski, Nebojša; Vikić- Topić, Dražen; Lauc, Gordan; Vujčić, Zoran; Josić, Djuro
Qualitative and quantitative NMR in optimization of methyl β-D-fructofuranoside synthesis catalyzed by invertase glycoforms // 21st Annual Central European NMR Symposium & Bruker users meeting
Beograd, Srbija, 2019. str. 62-62 (poster, međunarodna recenzija, sažetak, ostalo)


CROSBI ID: 1024064 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Qualitative and quantitative NMR in optimization of methyl β-D-fructofuranoside synthesis catalyzed by invertase glycoforms

Autori
Andjelković, Uroš ; Gudelj, Ivan ; Klarić, Thomas ; Hinneburg, Hannes ; Vinković, Marijana ; Wittine, Karlo ; Dovezenski, Nebojša ; Vikić- Topić, Dražen ; Lauc, Gordan ; Vujčić, Zoran ; Josić, Djuro

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Skup
21st Annual Central European NMR Symposium & Bruker users meeting

Mjesto i datum
Beograd, Srbija, 04.09.2019. - 05.09.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
N- glycosylation ; enzyme stability ; glycoform selection ; organic solvent ; methyl β-D-fructofuranoside ; enzymatic alkylation

Sažetak
Glycoforms of invertase that exhibit high stability in hydrophilic organic solvent was purified from a crude mixture of a multitude of invertase N-glycoforms synthesized by Saccharomyces cerevisiae. Purification was achieved by specially designed sub- fractionation procedure on anion-exchange chromatographic monolithic supports. Among separated N-glycoforms (EINV1-EINV4), only N- glycoform EINV1 exhibited 2-5 times higher stability in 30% methanol. Difference of glycoforms stability in the presence of high methanol concentration was used to improve the efficiency and yield of methyl β-D- fructofuranoside (MF) synthesis. Formation of MF in the reaction mixtures was confirmed by COSY, HMBC and HMQC. Kinetic of MF formation was assessed by quantitative NMR. NMR analysis provided data that enabled identification of optimal reaction time and calculation of reaction yield. The efficiency and yield of MF synthesis was improved more than 50% when the most stabile N- glycoform was utilized. These data underline the importance of analysis of glycan structures attached to glycoproteins, demonstrates different impact of N-glycans on invertase stability in hydrophilic organic solvent reaction environment, and provide a platform for improvement of yield of biocatalytic processes by selection of N- glycoforms.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Interdisciplinarne prirodne znanosti, Biotehnologija



POVEZANOST RADA


Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Institut "Ruđer Bošković", Zagreb,
GENOS d.o.o.,
Sveučilište u Rijeci - Odjel za biotehnologiju

Citiraj ovu publikaciju:

Andjelković, Uroš; Gudelj, Ivan; Klarić, Thomas; Hinneburg, Hannes; Vinković, Marijana; Wittine, Karlo; Dovezenski, Nebojša; Vikić- Topić, Dražen; Lauc, Gordan; Vujčić, Zoran; Josić, Djuro
Qualitative and quantitative NMR in optimization of methyl β-D-fructofuranoside synthesis catalyzed by invertase glycoforms // 21st Annual Central European NMR Symposium & Bruker users meeting
Beograd, Srbija, 2019. str. 62-62 (poster, međunarodna recenzija, sažetak, ostalo)
Andjelković, U., Gudelj, I., Klarić, T., Hinneburg, H., Vinković, M., Wittine, K., Dovezenski, N., Vikić- Topić, D., Lauc, G., Vujčić, Z. & Josić, D. (2019) Qualitative and quantitative NMR in optimization of methyl β-D-fructofuranoside synthesis catalyzed by invertase glycoforms. U: 21st Annual Central European NMR Symposium & Bruker users meeting.
@article{article, author = {Andjelkovi\'{c}, Uro\v{s} and Gudelj, Ivan and Klari\'{c}, Thomas and Hinneburg, Hannes and Vinkovi\'{c}, Marijana and Wittine, Karlo and Dovezenski, Neboj\v{s}a and Viki\'{c}- Topi\'{c}, Dra\v{z}en and Lauc, Gordan and Vuj\v{c}i\'{c}, Zoran and Josi\'{c}, Djuro}, year = {2019}, pages = {62-62}, keywords = {N- glycosylation, enzyme stability, glycoform selection, organic solvent, methyl β-D-fructofuranoside, enzymatic alkylation}, title = {Qualitative and quantitative NMR in optimization of methyl β-D-fructofuranoside synthesis catalyzed by invertase glycoforms}, keyword = {N- glycosylation, enzyme stability, glycoform selection, organic solvent, methyl β-D-fructofuranoside, enzymatic alkylation}, publisherplace = {Beograd, Srbija} }
@article{article, author = {Andjelkovi\'{c}, Uro\v{s} and Gudelj, Ivan and Klari\'{c}, Thomas and Hinneburg, Hannes and Vinkovi\'{c}, Marijana and Wittine, Karlo and Dovezenski, Neboj\v{s}a and Viki\'{c}- Topi\'{c}, Dra\v{z}en and Lauc, Gordan and Vuj\v{c}i\'{c}, Zoran and Josi\'{c}, Djuro}, year = {2019}, pages = {62-62}, keywords = {N- glycosylation, enzyme stability, glycoform selection, organic solvent, methyl β-D-fructofuranoside, enzymatic alkylation}, title = {Qualitative and quantitative NMR in optimization of methyl β-D-fructofuranoside synthesis catalyzed by invertase glycoforms}, keyword = {N- glycosylation, enzyme stability, glycoform selection, organic solvent, methyl β-D-fructofuranoside, enzymatic alkylation}, publisherplace = {Beograd, Srbija} }




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