Pregled bibliografske jedinice broj: 1024064
Qualitative and quantitative NMR in optimization of methyl β-D-fructofuranoside synthesis catalyzed by invertase glycoforms
Qualitative and quantitative NMR in optimization of methyl β-D-fructofuranoside synthesis catalyzed by invertase glycoforms // 21st Annual Central European NMR Symposium & Bruker users meeting
Beograd, Srbija, 2019. str. 62-62 (poster, međunarodna recenzija, sažetak, ostalo)
CROSBI ID: 1024064 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Qualitative and quantitative NMR in
optimization of methyl β-D-fructofuranoside
synthesis catalyzed by invertase glycoforms
Autori
Andjelković, Uroš ; Gudelj, Ivan ; Klarić, Thomas ; Hinneburg, Hannes ; Vinković, Marijana ; Wittine, Karlo ; Dovezenski, Nebojša ; Vikić- Topić, Dražen ; Lauc, Gordan ; Vujčić, Zoran ; Josić, Djuro
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo
Skup
21st Annual Central European NMR Symposium & Bruker users meeting
Mjesto i datum
Beograd, Srbija, 04.09.2019. - 05.09.2019
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
N- glycosylation ; enzyme stability ; glycoform selection ; organic solvent ; methyl β-D-fructofuranoside ; enzymatic alkylation
Sažetak
Glycoforms of invertase that exhibit high stability in hydrophilic organic solvent was purified from a crude mixture of a multitude of invertase N-glycoforms synthesized by Saccharomyces cerevisiae. Purification was achieved by specially designed sub- fractionation procedure on anion-exchange chromatographic monolithic supports. Among separated N-glycoforms (EINV1-EINV4), only N- glycoform EINV1 exhibited 2-5 times higher stability in 30% methanol. Difference of glycoforms stability in the presence of high methanol concentration was used to improve the efficiency and yield of methyl β-D- fructofuranoside (MF) synthesis. Formation of MF in the reaction mixtures was confirmed by COSY, HMBC and HMQC. Kinetic of MF formation was assessed by quantitative NMR. NMR analysis provided data that enabled identification of optimal reaction time and calculation of reaction yield. The efficiency and yield of MF synthesis was improved more than 50% when the most stabile N- glycoform was utilized. These data underline the importance of analysis of glycan structures attached to glycoproteins, demonstrates different impact of N-glycans on invertase stability in hydrophilic organic solvent reaction environment, and provide a platform for improvement of yield of biocatalytic processes by selection of N- glycoforms.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Interdisciplinarne prirodne znanosti, Biotehnologija
POVEZANOST RADA
Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Institut "Ruđer Bošković", Zagreb,
GENOS d.o.o.,
Sveučilište u Rijeci - Odjel za biotehnologiju
Profili:
Dražen Vikić-Topić (autor)
THOMAS STEPHEN KLARIĆ (autor)
Ivan Gudelj (autor)
Marijana Vinković (autor)
Karlo Wittine (autor)
Gordan Lauc (autor)
Đuro Josić (autor)