Naslov
IgG N-glycosylation is a potential biomarker for sepsis

Autori
Keser, Toma ; Pavić, Tamara ; Fressl Juroš, Gordana ; Lauc, Gordan ; Gornik Olga

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology "Crossroads in Life Sciences" (HDBMB2019)

Mjesto i datum
Lovran, Hrvatska, 25.09.2019. - 28.09.2019

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
sepsis ; N-glycans ; IgG ; septic shock ; biomarker

Sažetak
Sepsis is defined as infection with organ dysfunction due to a dysregulated immune response. It is a potentially fatal condition and its clinical manifestations vary with a rapid progression. Due to the existence of non- infectious systemic inflammatory response syndrome (SIRS) in many critical patients, how to differentiate sepsis from the non-infectious SIRS at the early stage has become a burning issue. The clinical values of many existing biomarkers are still uncertain or controversial. Therefore, we investigated a potential diagnostic and prognostic value of IgG N-glycosylation in 72 patients who underwent abdominal surgery, of which 36 developed sepsis. The blood samples were taken few hours after the surgery, IgG was isolated, N-glycans released with PNGase F and the labelled purified N-glycans were analysed with HILIC-UHPLC-FLR. The data analysis showed that patients who developed sepsis had a decrease in neutral glycan structures with bisecting N- acetylglucosamine at the time of sampling. Furthermore, patients who developed septic shock had an increase in monosialylated digalactosylated IgG N-glycan and a decrease in general IgG core fucosylation. We also found a strong association between IgG N-glycome and presepsin (sCD14-subtypes), a novel sepsis biomarker. Additionally, we compared the sepsis prediction performance of IgG N-glycome and presepsin, using a regularized logistic regression model. The IgG N-glycome showed a significantly better prediction performance (AUC=0.933) compared to presepsin (AUC=0.738). Altogether, we conclude that IgG N- glycosylation has a great potential to become a novel biomarker for sepsis, although further replication studies with a bigger sample size are required to confirm this.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Farmacija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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