Pregled bibliografske jedinice broj: 1022467
Effect of Tff3 Deficiency and ER Stress in the Liver
Effect of Tff3 Deficiency and ER Stress in the Liver // International journal of molecular sciences, 20 (2019), 18; 4389, 19 doi:10.3390/ijms20184389 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1022467 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Effect of Tff3 Deficiency and ER Stress in the Liver
Autori
Šešelja, Kate ; Bazina, Iva ; Welss, Jessica ; Schicht, Martin ; Paulsen, Friedrich ; Bijelić, Nikola ; Rođak, Edi ; Horvatić, Anita ; Gelemanović, Andrea ; Mihalj, Martina ; Baus Lončar, Mirela
Izvornik
International journal of molecular sciences (1422-0067) 20
(2019), 18;
4389, 19
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
ER stress ; trefoil peptide 3 ; liver ; tunicamycin ; proinflammatory cytokines
Sažetak
Endoplasmic reticulum (ER) stress, a cellular condition caused by the accumulation of unfolded proteins inside the ER, has been recognized as a major pathological mechanism in a variety of conditions, including cancer, metabolic and neurodegenerative diseases. Trefoil factor family (TFFs) peptides are present in different epithelial organs, blood supply, neural tissues, as well as in the liver, and their deficiency has been linked to the ER function. Complete ablation of Tff3 expression is observed in steatosis, and as the most prominent change in the early phase of diabetes in multigenic mouse models of diabesity. To elucidate the role of Tff3 deficiency on different pathologically relevant pathways, we have developed a new congenic mouse model Tff3-/-/C57BL6/N from a mixed background strain (C57BL6/N /SV129) by using a speed congenics approach. Acute ER stress was evoked by tunicamycin treatment, and mice were sacrificed after 24 h. Afterwards the effect of Tff3 deficiency was evaluated with regard to the expression of relevant oxidative and ER stress genes, relevant proinflammatory cytokines/chemokines, and the global protein content. The most dramatic change was noticed at the level of inflammation-related genes, while markers for unfolded protein response were not significantly affected. Ultrastructural analysis confirmed that the size of lipid vacuoles was affected as well. Since the liver acts as an important metabolic and immunological organ, the influence of Tff3 deficiency and physiological function possibly reflects on the whole organism.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
POVEZANOST RADA
Projekti:
HRZZ-IP-2016-06-2717 - TFF3 protein na raskrižju metabolizma i neurodegeneracije (Inter MeNe-3) (Baus Lončar, Mirela, HRZZ - 2016-06) ( CroRIS)
Ustanove:
Veterinarski fakultet, Zagreb,
Institut "Ruđer Bošković", Zagreb,
Medicinski fakultet, Osijek
Profili:
Mirela Baus Lončar
(autor)
Edi Rođak
(autor)
Andrea Gelemanović
(autor)
Nikola Bijelić
(autor)
Iva Bazina
(autor)
Martina Mihalj
(autor)
Anita Horvatić
(autor)
Kate Šešelja
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE