Naslov
Lipogenesis and lipid peroxidation in high- fructose and cafeteria diet rodent models

Autori
Mašek, Tomislav ; Roškarić, Petra ; Maurić, Maja ; Barišić, Josip ; Šperanda, Marcela ; Severin, Krešimir ; Starčević, Kristina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Congress Proceedings: 23rd Congress of the European Society of Veterinary and Comparative nutrition / - , 2019, 152-152

ISBN
979-12-200-5280-1

Skup
23rd Congress of the European Society of Veterinary and Comparative Nutrition

Mjesto i datum
Torino, Italija, 18.09.2019. - 20.09.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Lipids, Fructose, Cafeteria diet

Sažetak
Introduction. High fructose and cafeteria diet are extensively used rodent models in the investigations of metabolic syndrome [1]. Although interchangeably used, these models have specific differences in lipogenesis [2]. Therefore, our aim was to investigate the differences in the fatty acid profile and the extent of lipid peroxidation in these rodent models as well as the influence of sex. Material and Methods. Thirty Wistar rats (15 male and 15 female) were divided into control group (CON), high fructose group (HF, 15% of fructose in the drinking water) and cafeteria diet group (CAF) which was made by mixing basal diet and cafeteria diet in 1:1 ratio [3]. The animals were kept in polycarbonate cages and fed with experimental diets over a period of 16 weeks. The analysis of the hepatic fatty acid composition was performed using gas chromatography [4]. Malondialdehyde concentration (MDA) was determined using high performance liquid chromatography and 4- hydroxynonenal (HNE) concentration was determined using western blotting. For statistical data analysis, GraphPad 8 was used. Data were compared by analysis of variance and Tukey post hoc test. Results and discussion. The fatty acid profile and lipid peroxidation significantly differed between high fructose and cafeteria diet. In general, cafeteria diet caused significant loss of n3 fatty acids, especially EPA and increase in n6 fatty acids, especially C22:5n6. Lipid peroxidation measured by MDA was increased in CAF in both male and female in comparison to HF and CON. HNE concentration was significantly increased in HF male group.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA