Naslov
Protein alterations in response to endurance race, a proteomic study

Autori
Brkljača Bottegaro, Nika ; Gotić, Jelena ; Vrbanac, Zoran ; Horvatić, Anita ; Kuleš, Josipa ; Gelemanović, Andrea ; David, Eckersall ; Mrljak, Vladimir ;

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
6th VEPRA / 1st ECVSMR Conference Proceedings / Samoy, Yves ; Vrbanac, Zoran - Ghent : KanVet Resources for Veterans, 2019, 130-130

ISBN
978-83-933750-2-8

Skup
1th ECVSMR and 6th VEPRA conference

Mjesto i datum
Gent, Belgija, 19.09.2019. - 21.09.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
horse, endurance, proteomics

Sažetak
Strenuous exercise induces a variety of physiological adaptations in different body systems. The proteomic analysis enables an identification of different biological pathways interconnected with changes in proteome due to the exercise. The aim of the study was to evaluate changes in protein levels during an endurance race to highlight physiological adaptation to prolonged aerobic exercise. Thirteen horses, Arabian and Arabian cross, that successfully finished an 80 km endurance race have been enrolled in the study. Blood samples were collected before and 30 minutes after the race. Protein alterations in equine serum samples were analysed using the high- resolution tandem mass tag (TMT)-based quantitative proteomics by employing an UltiMate 3000 RSLCnano system coupled to a Q Exactive Plus mass spectrometer. Significant quantitative changes in protein concentration after the race were noted in levels of fibronectin, actin, and apolipoprotein. While fibronectin and actin were upregulated, apolipoprotein was downregulated after the race. Changes in fibronectin levels could be attributed to biomechanical loading during exercise and subsequently induced cartilage extracellular matrix changes. Apolipoprotein is a protein involved in energy supply through lipid metabolism and subsequent hypertriglyceridemia emphasising the importance of fatty acid aerobic metabolism in endurance horses. Changes in actin levels in response to endurance race could be related to muscle remodelling as a process of skeletal muscle adaptation to strenuous exercise. The preliminary results of the present study indicate that prolonged submaximal aerobic exercise during endurance race affects proteins partaking in metabolic pathways of articular cartilage and lipid utilisation.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA