Naslov
Markers of oxidative stress in search and rescue dogs during fieldwork

Autori
Vrbanac, Zoran ; Brkljača Bottegaro, Nika ; Šuran, Jelena ; Bureš, Tomislav ; Šmit, Iva ; Radić, Božo ; Brozić, Diana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
6th VEPRA / 1st ECVSMR Conference Proceedings / Samoy, Yves ; Vrbanac, Zoran - Ghent : KanVet Resources for Veterans, 2019, 142-142

ISBN
978-83-933750-2-8

Skup
6th VEPRA / 1st ECVSMR Conference

Mjesto i datum
Gent, Belgija, 19.09.2019. - 21.09.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
exercise, search and rescue dogs, oxidative stress

Sažetak
Exercise can result in oxidative stress characterised by an imbalance between reactive oxygen species (ROS) and antioxidants what can have a negative influence on homeostasis. The aim of the study was to evaluate oxidative stress in Croatian Mountain Rescue Service dogs after a simulated field search exercise. Eighteen healthy dogs of different breeds, mean age 3.05 (±1.85) years were included in this study. Two blood samples were taken from the dogs, early morning before and immediately after the 6 hours fieldwork. The analysis consisted of determination of two serum indicators of oxidative stress - reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) - and two erythrocytes antioxidant enzymes- glutathione peroxidase (GPx) and superoxide dismutase (SOD). Significant decreases (p<0.05) in serum level of d-ROMs and erythrocyte activity of SOD were recorded after the fieldwork. A decreasing trend in BAP level and GPx activity was also observed post activity, but not statistically significant. Increases in SOD and GPx enzyme activity in erythrocytes corresponds with enhanced resistance to oxidative stress. Our study demonstrated decreases in SOD activity, which could indicate the reduction in enzymatic antioxidant activity. BAP represents total antioxidant potential, while dROMS measure pro- oxidant status, and can give an estimate on potential ROS production. In trained individuals, due to repetitive endurance training, oxidative stress markers remain unaltered. The conclusion of our study is that a proper moderate intensity, long duration exercise in search and rescue dogs could lead to conditioning and improvement on the canine organism to respond to oxidative stress.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA