Naslov
Changes in IgG glycosylation profile in HFRS patients infected with Puumala Virus

Autori
Markotić, Alemka ; Krištić, Jasminka ; Kurolt, Ivan Christian ; Tijardović, Marko ; Cvetko Krajinović, Lidija ; Mačak Šafranko, Željka ; Svoboda, Petra ; Lauc, Gordan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts - 11th International Conference on Hantaviruses / - , 2019, 46-46

Skup
11th International Conference on Hantaviruses (ICH 2019)

Mjesto i datum
Leuven, Belgija, 01.09.2019. - 04.09.2019

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Hemorrhagic fever with renal syndrome, HILIC-UHPLC, inflammation

Sažetak
Hemorrhagic fever with renal syndrome (HFRS) is caused by Orthohantaviruses (HTV), which belonging to genus Orthohantavirus. Puumala orthohantavirus (PUUV) is pathogenic HTV, which predominantly cause mild to moderate HFRS in Croatia and in the rest of Europe. Inflammation is one of the hallmarks of the HFRS immunopathogenesis. The aim of our study is that we analyze possible changes in the glycosylation profile of IgG in HFRS patients during the course of disease, in order to better understand its potential influence on inflammation and other immunopathogenetic mechanisms. It is the first such study conducted in HFRS patients. Study was conducted in adult patients with confirmed HFRS, hospitalized at the University Hospital for Infectious Diseases, Zagreb, Croatia. Two sera samples (on admission and dismission) from 68 HFRS patients, were tested by the Hydrophilic Interaction Liquid Chromatography-Ultra High Performance Liquid Chromatography (HILIC-UHPLC) to determine the glycosylation profile of IgG in the serum of all subjects. The results were compared with the glycosylation profile of 17 controls, which were matched by age and sex. We used a linear mixed model to analyze N-glycoprotein changes in time. We analyzed each of the three groups individually. P values were corrected for multiple assays using the Benjamini-Hochberg method. We found that the level of glycans without galactose (G0) is significantly higher and the level of glycans with two galactose (G2) and the level of glycans with sialic acid (S) is lower in point 2 (end of treatment) than in point 1 (treatment initiation). In addition, at point 1 we detected less core fucosylated glycans (F) and more glycans with bisecting GlcNAc (B) than in point 2. Our findings indicate that changes in the glycosylation profile of IgG, in sera of HFRS patients, may have influence on dynamic of inflammatory process during the course of disease and on antibody-dependent cell-mediated cytotoxicity (ADCC). Further analysis of clinical and other laboratory parameters and potential correlation with glycosylation changes are in process.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Kliničke medicinske znanosti, Farmacija

POVEZANOST RADA