Naslov
Immunohistochemical lymphocytic profile of gingival tissue in orthodontic patients with allergy on titanium and/or nickel

Autori
Žigante, Martina ; Matušan Ilijaš, Koviljka ; Prpić, Jelena ; Špalj, Stjepan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts 2019 BAOS / - , 2019, 114-114

Skup
3rd Congress of Balkan Association of Orthodontic Specialists & 4th Congress of Macedonian Orthodontic Society: “The Highs and Lows of Orthodontics – Our Learning Curve”

Mjesto i datum
Ohrid, Sjeverna Makedonija, 12.09.2019. - 15.09.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Allergy ; Titanium ; Nickel ; Lymphocytes ; Immunohistochemistry

Sažetak
AIM: Due to oral corrosion of alloys during orthodontic treatment allergic reactions on titanium and/or nickel can occur. Mechanisms of delayed-type hypersensitivity reactions are mostly studied in allergic contact dermatitis and allergic reaction to orthopaedic implant metals but not in orthodontic patients. Delayed type of hypersensitivity reactions are mediated by antigen-specific T lymphocytes. Th1 cells control cellular immunity by recognizing antigens and releasing IFN-y to activate effector cells. Even though delayed type of hypersensitivity is mostly related to Th1 lymphocytes other phenotypic T cells can also be involved such as cytotoxic lymphocytes. The aim was to analyze the immunohistochemical profile of lymphocytic infiltrate in gingival tissue of patients undergoing orthodontic treatment and to compare it to patients’ titanium and/or nickel allergy status. Materials and Methods: External gingivectomy of enlarged gingiva was performed. Immunohistochemical profile of lymphocytic infiltrate was analyzed using antibodies against CD3 (lymphocytes T) CD20 (lymphocytes B) CD4 (Th1 lymphocytes) and CD8 (cytotoxic lymphocytes) molecules on 26 formalin fixed and paraffin embedded gingival tissue samples. Seven patients had confirmed allergy on titanium and/or nickel and 19 were non-allergic and used as control group. The density of a particular lymphocyte type was determined as a percentage of the surface of the stroma where the infiltrate was present using x10 objective lens. Results: There was a tendency towards an increase in CD3 CD4 and CD20 while a decrease in CD8 in allergic patients. Also increased CD3/CD20 ratio and decreased CD4/CD8 ratio is observed in allergic patients. But the differences were not statistically significant. Conclusion: Results indicate that gingival tissue reaction to titanium and/or nickel engages more T than B lymphocytes and also more helper lymphocytes than cytotoxic ones.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Dentalna medicina

POVEZANOST RADA