Pregled bibliografske jedinice broj: 1020522
Neurodegeneration, microglyosis and astrocytosis in the optic tract during the first week following repetitive mild traumatic brain injury in wild type and TDP-43 transgenic mice
Neurodegeneration, microglyosis and astrocytosis in the optic tract during the first week following repetitive mild traumatic brain injury in wild type and TDP-43 transgenic mice // 7th Croatian Neuroscience Congress, Book of abstracts
Zadar, Hrvatska, 2019. str. 55-55 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1020522 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Neurodegeneration, microglyosis and astrocytosis in the optic tract during the first week following repetitive mild traumatic brain injury in wild type and TDP-43 transgenic mice
Autori
Rajič Bumber, Jelena ; Pilipović, Kristina ; Gržeta, Nika ; Dolenec, Petra ; Janković, Tamara ; Križ, Jasna ; Župan, Gordana
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
7th Croatian Neuroscience Congress, Book of abstracts
/ - , 2019, 55-55
Skup
7th Croatian Neuroscience Congress
Mjesto i datum
Zadar, Hrvatska, 12.09.2019. - 15.09.2019
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
repetitive mild traumatic brain injury, TDP-43, neurodegeneration, microglia, astrocytes, mice
Sažetak
Introduction: Repetitive mild traumatic brain injury (mTBI) is associated with the development of neurodegenerative diseases such as amyotrophic lateral sclerosis and chronic traumatic encephalopathy in which TAR DNA- binding protein 43 (TDP-43) dysregulation has been identified. The purpose of this study was to investigate if repetitive mTBI induces neurodegeneration and the changes in microglia and astrocytes in the brain during the first week after the repetitive mTBI in wild type and mice overexpressing mutant TDP-43. Materials and methods: Repetitive mTBI in C57BL/6 and TDP-43G348C (Swarup et al., 2011) mice was induced by using weight drop method (Kane et al. 2012) for five days in a row, twice daily, at intervals of six hours. Sham- injured mice of the control wild type or transgenic TDP-43 mice groups were anesthetized without receiving any injury. The animals were sacrificed 1, 3 or 7 days after the final injury or sham procedure and their brains were prepared for histological analyses. Fluoro-Jade C staining was used to identify the extent of neuronal injury. Immunofluorescence labelings of glial fibrillary acidic protein (GFAP) and ionized calcium binding adaptor molecule 1 (Iba1) were performed with the aim to assess the expressions of astrocytes and microglial cells. Results: Fluoro-Jade C positive fibers as well as increased GFAP and Iba1 immunoreactivity were detected in the optic tracts of the injured wild type and TDP-43 transgenic mice at days 1, 3 and 7 after the last rmTBI. Conclusion: This preliminary study suggests the presence of neurodegenerative changes as well as of astrocytosis and microgliosis in the optic tract at early time points following repetitive mTBI in wild type and TDP43 transgenic mice. This research was fully supported by the Croatian Science Foundation under the project IP-2016-06-4602 to Ž.G.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti, Kognitivna znanost (prirodne, tehničke, biomedicina i zdravstvo, društvene i humanističke znanosti)
POVEZANOST RADA
Projekti:
HRZZ-IP-2016-06-4602 - Blaga repetitivna traumatska ozljeda mozga: model za istraživanje neuropatologije i neuroupale posredovane s TDP-43 (TDP-43repTBI) (Župan, Gordana, HRZZ - 2016-06) ( CroRIS)
Ustanove:
Medicinski fakultet, Rijeka
Profili:
Petra Dolenec
(autor)
Jelena Rajič Bumber
(autor)
Gordana Župan
(autor)
Tamara Janković
(autor)
Jasna Križ
(autor)
Kristina Pilipović
(autor)
Nika Gržeta
(autor)