Pregled bibliografske jedinice broj: 1017502
Cellular responses to proteome-wide isoleucine mistranslation
Cellular responses to proteome-wide isoleucine mistranslation // EMBO Practical Course, Quantitative Proteomics: Strategies and Tools to Probe Biology
Heidelberg, Njemačka, 2019. (poster, nije recenziran, neobjavljeni rad, znanstveni)
CROSBI ID: 1017502 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Cellular responses to proteome-wide isoleucine
mistranslation
Autori
Pranjić, Marija ; Močibob, Marko ; Šemanjski, Maja ; Maček, Boris ; Gruić Sovulj, Ita
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni
Skup
EMBO Practical Course, Quantitative Proteomics: Strategies and Tools to Probe Biology
Mjesto i datum
Heidelberg, Njemačka, 05.05.2019. - 10.05.2019
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
canonical and noncanonical mistranslation ; DnaK ; quantitative proteomics
Sažetak
We investigated an effect of two different types of substitutions: canonical (Ile-to-Val) and noncanonical (Ile-to-Nva) on the E. coli proteostasis machinery. We investigated cellular responses to mistranslation by in vivo experiments and with methods of quantitative proteomics to identify changes in the proteome and DnaK clients isolated in pull-down assay. Proteome analysis revealed significant upregulation of several chaperone systems but not proteases. However, mistranslation did not change the identity of DnaK clients and no noticeable difference between cellular responses to canonical and noncanonical mistranslation was observed.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Biologija
POVEZANOST RADA
Projekti:
HRZZ-IP-2016-06-6272 - Aminoacil-tRNA-sintetaze kao čuvari standardnog genetičkog koda (AARSCODE) (Gruić Sovulj, Ita, HRZZ - 2016-06) ( CroRIS)
Ustanove:
Prirodoslovno-matematički fakultet, Zagreb
