Naslov
Effect of estrogen deficiency on human serum and IgG N-glycosylation

Autori
Jurić, Julija ; Kohrt, Wendy M. ; Kifer, Domagoj ; Pezer, Marija ; Nigrovic, Peter A. ; Lauc, Gordan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Glycoconjugate Journal, Volume 36, Number 4 / Sonnino, Sandro - Milano : Springer, 2019, 329-329

Skup
International Symposium on Glycoconjugates

Mjesto i datum
Milano, Italija, 25.08.2019. - 31.08.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
estrogen, serum, IgG, N-glycosylation, glycans

Sažetak
Most proteins in human serum are glycosylated by diverse glycan structures that fine-tune their function. Regulatory role of glycans has been most extensively explored in immunoglobulin G (IgG). Different glycoforms of this antibody initiate versatile immune responses. It is known that diseases and aging alter N-glycosylation of IgG, but recently, sex hormones also emerged as a significant regulatory factor. Major differences in IgG N- glycosylation were reported in females during pregnancy, late puberty, and with entering the menopause. However, current knowledge of the associations between female sex hormones and IgG N-glycosylation is mostly based on epidemiological studies. Here we present an experimental study aiming to investigate the effect of menopause-like estrogen levels on N- glycosylation of IgG and total human serum proteins. Participants were healthy premenopausal women with normal menstrual cycle function, deprived of estrogen with monthly injections of gonadotropin-releasing hormone agonist (GnRHAG). During GnRHAG therapy, one group was given estrogen supplementation in the form of estradiol (E2) patches and the other group received a placebo. The study terminated with the spontaneous recovery of normal menstrual cycle function. Each participant was tested 3 times during the study. Each time, 5 ml of peripheral blood was drawn and serum was obtained. Additionally, IgG was isolated from sera by affinity chromatography on a protein G monolithic plate. Serum and IgG N-glycans were released by PNGase F, labeled with 2-AB and analyzed by hydrophilic interaction ultra- performance liquid chromatography (HILIC-UPLC). Our study indicates that circulating estrogen levels associated with serum and IgG N- glycosylation. Changes of N-glycosylation detected in serum most likely originate from IgG. Our results show that estrogen-deficient hormonal milieu causes higher bisection, and lower galactosylation and sialylation level that is usually involved in the proinflammatory background.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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