Naslov
Antimicrobial resistance of Yersinia enterocolitica O:3 isolated from tonsils and lymph nodes of slaughtered pigs in Croatia

Autori
Zdolec, Nevijo ; Pažin, Valerij ; Kiš, Marta

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Proceeding 13th SafePork 2019- Simposium on Epidemiology and Control of Biological, Chemical and Physical Hazards in the Pork Chain / - Berlin : AMP Association for Molecular Pathology, 2019, 169-170

ISBN
978-3-000635-07-6

Skup
13th Simposium on Epidemiology and Control of Biological, Chemical and Physical Hazards in the Pork Chain (SafePork 2019)

Mjesto i datum
Berlin, Njemačka, 26.08.2019. - 29.08.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Yersinia enterocolitica, AMR, pigs, tonsils, lymph nodes, VPH

Sažetak
Antimicrobial resistance of foodborne pathogens presents one of the main public health concerns. In this survey, phenotypic antimicrobial resistance was evaluated in Yersinia enterocolitica isolated from tonsils and lymph nodes of slaughtered pigs in Croatian slaughterhouses. Presumptive colonies (n=49) were selected from CIN and CHROMagarTM Y.enterocolitica and subjected to MALDI-TOF MS identification and serotyping. Antimicrobial susceptibility was tested by disk diffusion method toward levofloxacin, ciprofloxacin, ampicillin, cephalothin, cefotaxime, tetracycline, nalidixic acid, ceftazidime, trimethoprim/sulfamethoxazole, chloramphenicol and streptomycin. Resistance of strains was assessed following EUCAST/CLSI guidelines. All strains were confirmed by MALDI-TOF MS as Yersinia enterocolitica and belonged to O:3 serotype. The majority of strains was resistant toward ampicillin (91.6 %) and cephalothin (85.4 %), followed by chloramphenicol (31.2 %), nalidixic acid (31.2 %), streptomycin (27.0 %), tetracycline (8.3%) and trimethoprim/sulfamethoxazole (2.0 %). Only one strain was susceptible to all antimicrobial agents tested. Y. enterocolitica strains of pigs from medium-scale farms were mostly resistant to ampicillin and cephalothin, while strains collected from large farms were additionally resistant to chloramphenicol, nalidixic acid and streptomycin. Multiresistance (resistance to three or more agents) was found in 17 strains (35.4 %). Higher prevalence of multiresistant Y. enterocolitica was evident in pigs originated from large farms.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

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