Naslov
Short-term high NaCl dietary intake changes leukocyte expression of VLA-4, LFA-1 and Mac-1 integrins in both healthy humans and Sprague- Dawley rats ; a comparative study

Autori
Mihalj, Martina ; Matić, Anita ; Mihaljević, Zrinka ; Barić, Lidija ; Stupin, Ana ; Drenjančević, Ines

Izvornik
Mediators of inflammation (0962-9351) 2019 (2019); 6715275, 18

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
high salt diet ; Sprague-Dawley rats ; humans ; LFA-1 ; VLA-4 ; Mac-1 integrins ; peripheral blood leukocytes

Sažetak
This study aimed to assess the effects of short-term high salt (HS) diet on the peripheral blood leukocytes (PBL) activation status in healthy rats and young human individuals. Distribution of PBL subpopulations and surface expression of integrins were determined using flow cytometry in 36 men and women on 7-days low-salt diet (<3.2g salt/day) immediately followed by 7-days HS diet (~14g salt/day), or in Sprague-Dawley (SD) rats (N=24) on 0.4% NaCl diet (aLS group) or 4% NaCl diet (aHS group) for 7 days. aHS group presented with increased frequency of granulocytes, while the frequency of lymphocytes was reduced. Although in humans HS diet reduced the expression of CD11b(act) integrin on lymphocytes, the frequency of CD11b(act)-bearing cells among all PBL subsets was increased. aHS group of rats exhibited increased expression of total CD11b/c in granulocytes and CD3 lymphocytes. Expression of CD11a was significantly reduced in all PBL subsets from human subjects and increased in aHS group. CD49d expression on all PBL subsets was significantly decreased in both human and rats. In human subjects, we found reduced frequencies of intermediate monocytes accompanied by reciprocal increase in classical monocytes. Present results suggest that short- term HS diet can alter leukocytes’ activation status and promote vascular low-grade inflammation.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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