Naslov
Probiotic activity of Bifidobacterium animalis subsp. lactis BB-12® in dehydrated wheat/rice cereal products

Autori
Leboš-Pavunc, A., Penava, L., Ranilović, J., Novak, J., Banić, M., Butorac, K., Petrović, E., Mihaljević-Herman, V., Bendelja, K., Savić Mlakar, A., Durgo, K., Kos, B., Šušković, J.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Skup
Power of microbes

Mjesto i datum
Sveti Martin na Muri, Hrvatska, 15.05.2019. - 18.05.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
probiotic activity, infants, dehydrated wheat/rice cereal products

Sažetak
Probiotic activity of Bifidobacterium animalis subsp. lactis BB-12® in dehydrated wheat/rice cereal products developed for infants older than 4 months was investigated. The number of live BB-12® cells in three samples was above the minimal required number of probiotic cells per gram of product (1x106 CFU/g) till the end of the product expiration dates. Significantly higher viable cell counts were obtained in simulated gastrointestinal tract conditions when probiotic culture BB-12® was applied in processed wheat/rice cereal products compared to cell count of pure lyophilized culture. Also, wheat/rice cereal matrices contributed to the BB-12® in vitro cholesterol assimilation ability and antibacterial activity. Adhesion properties of BB-12® strain to Caco-2 cell line and proteins of extracellular matrix and competitive exclusion of test-microorganisms were investigated, whereas adhesion of BB-12® strain to Caco-2 cell monolayer was measured by fluorescence microscopy. Among conditions tested (MOI= 10 ; 100, incubation time 1h, 4h, 12h), the highest ratio of 35 BB-12® cells per 100 Caco-2 cells, was obtained at MOI 100 and after 12 hours of incubation. Additionally, the occurrence of BB-12® strain adhered to Caco-2 cell line reduced the attachment of S. Typhimurium FP1 for approximately 1.5 log10 CFU/ml, and of E. coli 3014 for approximately 0.71 log10 CFU/ml. Hence, considering the beforehand gained adhesion pattern, proved by live cell imaging microscope together with exclusion capacity, adhesion to extracellular matrix proteins and coaggregation with examined pathogens, BB-12® probiotic strain has an impact on the adhesion ability and could downsize the negative effects of both strains, S. Typhimurium FP1 and E. coli 3014.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA