Pregled bibliografske jedinice broj: 1013453
Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart
Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart // Genes, 10 (2019), 6; 474, 11 doi:10.3390/genes10060474 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1013453 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Immunophenotyping of a Stromal Vascular
Fraction from Microfragmented Lipoaspirate Used
in Osteoarthritis Cartilage Treatment and Its
Lipoaspirate Counterpart
Autori
Polančec, Denis ; Zenić, Lucija ; Hudetz, Damir ; Borić, Igor ; Jeleč, Željko ; Rod, Eduard ; Vrdoljak, Trpimir ; Skelin, Andrea ; Plečko, Mihovil ; Turkalj, Mirjana ; Nogalo, Boro ; Primorac, Dragan
Izvornik
Genes (2073-4425) 10
(2019), 6;
474, 11
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
lipoaspirate ; microfragmentedlipoaspirate ; immunophenotyping ; endothelialprogenitors ; pericytes ; supra-adventitialadiposestromalcells
Sažetak
Osteoarthritis (OA) is a degenerative joint disease accompanied by pain and loss of function. Adipose tissue harbors mesenchymal stem/stromal cells (MSC), or medicinal signaling cells as suggested by Caplan (Caplan, 2017), used in autologous transplantation in many clinical settings. The aim of the study was to characterize a stromal vascular fraction from microfragmented lipoaspirate (SVF-MLA) applied for cartilage treatment in OA and compare it to that of autologous lipoaspirate (SVF-LA). Samples were first stained using a DuraClone SC prototype tube for the surface detection of CD31, CD34, CD45, CD73, CD90, CD105, CD146 and LIVE/DEAD Yellow Fixable Stain for dead cell detection, followed by DRAQ7 cell nuclear dye staining, and analyzed by flow cytometry. In SVF- LA and SVF-MLA samples, the following population phenotypes were identified within the CD45– fraction: CD31+CD34+CD73±CD90±CD105±CD146± endothelial progenitors (EP), CD31+CD34– CD73±CD90±CD105– CD146± mature endothelial cells, CD31–CD34– CD73±CD90+CD105–CD146+ pericytes, CD31– CD34+CD73±CD90+CD105–CD146+ transitional pericytes, and CD31–CD34+CD73highCD90+CD105– CD146– supra-adventitial-adipose stromalcells(SA- ASC). Theimmunophenotyping profile ofSVF-MLAwas dominatedby areduction of leukocytes and SA-ASC, and an increase in EP, evidencing a marked enrichment of this cell population in the course of adipose tissue microfragmentation. The role of EP in pericyte- primed MSC-mediated tissue healing, as well as the observed hormonal implication, is yet to be investigated.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti
POVEZANOST RADA
Ustanove:
Medicinski fakultet, Rijeka,
Medicinski fakultet, Zagreb,
Klinička bolnica "Sveti Duh",
Medicinski fakultet, Split,
Medicinski fakultet, Osijek,
Dječja bolnica Srebrnjak,
Hrvatsko katoličko sveučilište, Zagreb,
Sveučilište u Rijeci - Odjel za biotehnologiju,
Specijalna bolnica Sv. Katarina,
Fakultet za dentalnu medicinu i zdravstvo, Osijek
Profili:
Lucija Zenić (autor)
Damir Hudetz (autor)
Mirjana Turkalj (autor)
Andrea Skelin (autor)
Igor Borić (autor)
Dragan Primorac (autor)
Eduard Rod (autor)
Denis Polančec (autor)
Boro Nogalo (autor)
Željko Jeleč (autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
Uključenost u ostale bibliografske baze podataka::
- BIOSIS Previews (Biological Abstracts)