Naslov
PTGS2 and ITGB1 gene expression in canine melanoma

Autori
Medven Zagradišnik, Lidija ; Guillemin, Nicolas ; Gudan Kurilj, Andrea ; Šoštarić-Zuckermann, Ivan-Conrado ; Artuković, Branka ; Grabarević, Željko ; Hohšteter, Marko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Journal of Comparative Pathology Volume 166, January 2019, Page 124 / Day, Michael J. - : Elsevier, 2019, 124-124

Skup
Joint ESVP and ECVP Congress (The European Society of Veterinary Pathology/European College of Veterinary Pathologists)

Mjesto i datum
Cluj-Napoca, Rumunjska, 05.09.2019. - 08.09.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
PTGS2, ITGB1, melanoma, dog

Sažetak
Introduction: Cyclo-oxygenase and integrin pathways are involved in the relationship between tumour cells and their microenvironment and have an important role in tumour progression. Canine oral melanocytic tumours are more malignant than their dermal counterparts. The objective of this pilot study was to investigate expression of the prostaglandin-endoperoxide synthase 2 (PTGS2) and integrin beta 1 (ITGB1) genes in canine melanomas. Materials and Methods: A total of 34 samples were divided into two groups: formalin-fixed, paraffin wax-embedded (FFPE) (n = 17) and fresh frozen tissue (FF) (n = 17). Each group contained four oral and two dermal melanomas, one lymph node metastasis and 10 negative controls (n = 5 skin ; n = 5 oral mucosa). A diagnosis of melanoma was confirmed histopathologically and immunohistochemically with melan A. PTGS2 and ITGB1 gene expression was investigated with qPCR using the ΔΔCT method. The Wilcoxon and Student tests were performed for statistical analysis, depending on data distribution for each group. Results: Oral melanomas showed higher expression of PTGS2 (FC 4.11, P <0.05) and ITGB1 (FC 5.48, P <0.01) in FF and ITGB1 (FC 2.82, P <0.05) in FFPE. No differences were found in dermal melanoma. The results showed a tendency toward positive correlation in ITGB1 gene expression in oral melanoma between FF and FFPE extraction (r = 0.64, P <0.1). Conclusions: This study shows the significant impact of sample preservation method and tissue type on gene expression quantification. The possibility of gene analysis from FFPE from retrospective studies has been confirmed. The results of PTGS2 and ITGB1 analysis suggest a more malignant behaviour of oral melanomas compared with dermal counterparts.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

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