Naslov
Searching for Marija Krucifiksa Kozulić: A Targeted Capture MPS Approach

Autori
Sanchez, Sidney Gaston ; Bašić, Željana ; Kružić, Ivana , Primorac, Dragan ; Daniels- Higginbotham , Jennifer ; Marshall, Charla ; Anđelinović, Šimun ; Bosnar, Alan ; Petaros, Anja ; Čoklo, Miran ; Holland, Mitchell

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
11th ISABS Conference on Forensic and Anthropologic Genetics and Mayo Clinic Lectures in Individualized Medicine / Primorac, Dragan ; Schanfield, Moses ; Vuk-Pavlović, Stanimir ; Kayser, Manfred ; Ördög, Tamás - Zagreb : International Society for Applied Biological Sciences (ISABS), 2019, 306-306

ISBN
987-953-57695-3-8

Skup
11th ISABS Conference on Forensic and Anthropologic Genetics and Mayo Clinic Lectures in Individualized Medicine

Mjesto i datum
Split, Hrvatska, 17.06.2019. - 22.06.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Marija Krucifiksa Kozulić ; mtgenome capture approach

Sažetak
The search for Marija Krucifiksa Kozulić’s remains has been an ongoing effort that has required the skills of many experts in the field, including forensic molecular biologists, anthropologists, and pathologists. Sister Kozulić was a pious and charitable nun who dedicated herself to helping the poor and less unfortunate. Due to her generous and virtuous life, Kozulić is currently being considered for the sainthood by the Vatican, requiring the identification of her 20th century skeletal remains. Kozulić was buried in a tomb with her biological sister, Tereza Kozulić, along with the commingled remains of other nuns from the Society of Sisters of the Sacred Heart of Jesus in Rijeka, Croatia. Previous analysis of the mitochondrial (mt) genome and control region revealed high levels of bacterial contamination and only produced common haplotypes that prohibited discrimination between the remains analyzed. A whole mtgenome capture approach developed by the Armed Forces DNA Identification Laboratory (AFDIL) was applied to the remains prior to massively parallel sequencing (MPS). This method removed a majority of the bacterial contamination by isolating the target mtDNA with the use of biotinylated RNA baits and washing away any foreign DNA. Using this technique, two sets of right and left femurs were identified as having the same mtgenome haplotype with a shared site of heteroplasmy at position 13327A (28-29% vs 12-13%), as well as an unshared heteroplasmic site at position 12337.1C (32-34%). These samples were extracted in duplicate at the Pennsylvania State University as well as sent to AFDIL to confirm the findings, and in all cases identical results were obtained. Given the consistency of the DNA results and the fact that the sisters were the only maternally related individuals within that closed population, these two sets of skeletal remains are believed to belong to Sisters Marija and Tereza Kozulić. It is now up to experts in anthropology and pathology to determine which set of remains belongs to Sister Marija.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Etnologija i antropologija

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