Naslov
Free radical production in gastrointestinal tract cells induced by titanium and nickel ions from orthodontic alloys

Autori
Rinčić Mlinarić, Marijana ; Durgo, Ksenija ; Komes, Draženka ; Žigante, Martina ; Špalj, Stjepan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
European Journal of Orthodontics. Abstracts of Lectures and Scientific Posters.2019 ; 41:159-160. / - , 2019, 159-160

Skup
95th Congress of the European Orthodontic Society

Mjesto i datum
Nica, Francuska, 17.06.2019. - 22.06.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
oxidative stress ; free radicals ; corrosion ; nickel-titanium alloy

Sažetak
Aim: The oxidative stress in human cells of gastrointestinal tract in the presence of metallic ions released by corrosion of nickel- titanium (NiTi) alloy was investigated. Methods: The dynamics of nickel and titanium ions release from eighteen samples of NiTi archwires (0.020x0.020'') immersed in artificial saliva (pH 4.8, 37°C) in period of two weeks was recorded. Induction of free radicals was assessed in commercial cell lines of human tongue CAL 27, liver HEP G2 and colon Caco-2 after exposure to real concentration of corrosion products, 5x concentration and dilutions of 0.5x and 0.1x. Influence of each metal separately was performed by exposing cells to only nickel or only titanium ions. Cells were monitored for 24, 48 and 72 hours. Results: Significant increase in free radicals in all cell lines, after 24 and 48 hours, is present when concentration both metals is at least 162 µg/L and comprises of combination of 75.5 µg/L Ni and 86.5 µg/L Ti or 128 µg/L Ni and 44.9 µg/L Ti (p<0.001). The effect is lower after 72 hours. Shorter exposure induces more free radicals (24 hours - 30-100%) than longer exposure (48 and 72 hours - 10 to 40%). More oxidative stress is present in tongue cells. The concentration of 30.4 μg/L Ni and 17.3 μg/L Ti, that was the highest recorded in saliva during 14 days, or the concentration of both metals together with at least 42.3 μg/L induced mostly <50% of the radicals, both in shorter and longer exposure periods. Nickel and titanium are equally important predictors of radical production. Conclusion: Corrosion of NiTi alloys in low pH saliva induce higher oxidative stress in tongue cells than in liver and colon. Lower oxidative stress after longer exposure implies high reparatory ability of cells.

Izvorni jezik
Engleski

Znanstvena područja
Dentalna medicina, Biotehnologija

Napomena
Financirano projektom HRZZ 7500

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