Pregled bibliografske jedinice broj: 1007020
Design of PGLa-H tandem-repeat peptides with activity and selectivity testing against clinical bacterial isolates
Design of PGLa-H tandem-repeat peptides with activity and selectivity testing against clinical bacterial isolates // Book of Abstracts
Rouen, Francuska, 2016. str. 245-245 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1007020 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Design of PGLa-H tandem-repeat peptides with activity and selectivity testing against clinical bacterial isolates
Autori
Rončević, Tomislav ; Gajski, Goran ; Ilić, Nada ; Goić-Barišić, Ivana ; Tonkić, Marija ; Simunić, Juraj ; Juretić, Davor
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Book of Abstracts
/ - , 2016, 245-245
Skup
RegPep 2016
Mjesto i datum
Rouen, Francuska, 12.07.2016. - 14.07.2016
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Antimicrobial peptides ; PGLa-H ; Clinical isolates ; Multidrug resistant ; Cytoxicity
Sažetak
Antimicrobial peptides (AMPs) are promising candidates for new classes of antibiotics but tend to display an inacceptable toxicity to human cells. A naturally produced C-terminal fragment of PGLa, named PGLa-H, was reported to have a very low haemolytic activity with a moderate antibacterial activity. We designed of sequential tandem repeat of PGLa-H and its analogue with glycine substitution at key position. DiPGLa-H and its analogue showed markedly improved in vitro bacteriostatic and bactericidal activity against laboratory strains and multidrug resistant clinical isolates of both Gram-negative and Gram-positive pathogens. At the same time they were non-toxic for circulating human blood cells as assessed by geno/cytotoxicity and haemolysis assays. An analogue with a single glycine substitution showed a slightly better antibacterial activity and somewhat reduced cytotoxicity. In the future these peptides may serve as useful lead compounds for developing non-toxic anti-infective agents against multi-resistant pathogens, especially for topical uses.
Izvorni jezik
Engleski
Znanstvena područja
Fizika, Biologija, Interdisciplinarne prirodne znanosti
POVEZANOST RADA
Projekti:
HRZZ-IP-2013-11-8481 - Biofizikalni dizajn antimikrobnih peptida i inovativni molekularni deskriptori (BioAmpMode) (Vukičević, Damir) ( CroRIS)
Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb,
KBC Split,
Prirodoslovno-matematički fakultet, Split,
Medicinski fakultet, Split
Profili:
Tomislav Rončević
(autor)
Davor Juretić
(autor)
Goran Gajski
(autor)
Juraj Simunić
(autor)
Nada Ilić
(autor)
Ivana Goić Barišić
(autor)
Marija Tonkić
(autor)