Naslov
The role of regulatory T-lymphocytes in cows infected with bovine leukemia virus

Autori
Vlahović (Grabarević), Dunja ; Artuković, Branka ; Branović Čakanić, Karmen ; Grabarević, Željko ; Hohšteter, Marko ; Medven Zagradišnik, Lidija ; Šoštarić, Branko ; Šoštarić-Zuckermann, Ivan-Conrado ; Gudan Kurilj, Andrea

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
27th Ljudevit Jurak International Symposium on Comparative Pathology with One Health Session Final Program and Book of Abstracts / - , 2019, 48-48

Skup
27th Ljudevit Jurak International Symposium on Comparative Pathology

Mjesto i datum
Zagreb, Hrvatska, 31.05.2019. - 01.06.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Regulatory T-lymphocytes, enzootic bovine leukosis, bovine leukemia virus, cattle

Sažetak
THE ROLE OF REGULATORY T-LYMPHOCYTES IN COWS INFECTED WITH BOVINE LEUKEMIA VIRUS Vlahović, D.1, B. Artuković2, K. Branović Čakanić1, Ž. Grabarević2, M. Hohšteter2, L. Medven Zagradišnik2, B. Šoštarić1, I.-C. Šoštarić-Zuckermann2 and A. Gudan Kurilj2 1Department for Pathological Morphology, Croatian Veterinary Institute, Zagreb, Croatia and 2Department of Veterinary Pathology, University of Zagreb, Zagreb, Croatia Enzootic bovine leukosis is a chronic contagious disease of cattle caused by bovine leukemia virus (BLV), an oncogenic RNA Deltaretrovirus of Retroviridae family. It has an important economic impact because it causes reduction in milk production, increased culling rate, shorter longevity and increased susceptibility to secondary diseases. According to the recent literature data, regulatory T-lymphocytes play an important role in progression of viral diseases by suppressing responses of other immune cells. Their role in enzootic bovine leukosis is still not sufficiently investigated. The aim of this study was to compare number of Treg-lymphocytes in lymphatic organs of BLV-positive cows and BLV-negative cows in order to determine their potential role in pathogenesis of the disease. Tracheobronchial and mesenteric lymph nodes, spleen and blood samples were collected in slaughterhouse from 26 serologically BLV-positive and 10 BLV-negative cows as negative control. Listed organs were grossly examined before sampling for histopathological analysis and immunohistochemical staining for FOXP3+ Treg-lymphocytes. Hematological analysis of blood samples was performed. Since hematological analysis showed no increase in lymphocyte count and no lymphoma was observed on gross examination it was concluded that BLV-positive animals were in early stage of infection. In 13 BLV-positive cows, most common gross finding was enlarged tracheobronchial lymph node followed by enlarged mesenteric lymph node. Most common histopathological finding was reactive hyperplasia, mild in lymph nodes and moderate in spleen. Immunohistochemical staining showed higher average value of Treg-lymphocytes in tracheobronchial lymph nodes in BLV-positive cows (9, 05%) compared to control group (7, 06%). Higher average value of Treg-lymphocytes in tracheobronchial lymph nodes of BLV-positive cows indicates their potential immunosuppressive function during early stage of infection.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA