Naslov
Comparative proteomic study of phytotoxic effects of silver nanoparticles and silver ions on tobacco plants

Autori
Peharec Štefanić, Petra ; Jarnević, Martina ; Cvjetko, Petra ; Biba, Renata ; Šikić, Sandra ; Tkalec, Mirta ; Cindrić, Mario ; Letofsky-Papst, Ilse ; Balen, Biljana

Izvornik
Environmental Science and Pollution Research (0944-1344) 26 (2019); 22529-22550

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Silver nanoparticles ; Silver nitrate ; Nicotiana tabacum ; Two-dimensional electrophoresis ; Proteomics ; Phytotoxicity

Sažetak
Widespread application of silver nanoparticles (AgNPs), due to their antibacterial and antifungal properties, increasestheir release into the environment and potential detrimental impact on living organisms. Plants may serve as apotential pathway for AgNPs bioaccumulation and a route into the food chain, hence investigation of AgNP phy-totoxic effects are of particular importance. Since proteins are directly involved in stress response, studies of theirabundance changes can help elucidate the mechanism of the AgNP-mediated phytotoxicity. In this study, we inves-tigated proteomic changes in tobacco (Nicotiana tabacum) exposed to AgNPs and ionic silver (AgNO3). A highoverlap of differently abundant proteins was found in root after exposure to both treatments, while in leaf, almost ahalf of the proteins exhibited different abundance level between treatments, indicating tissue-specific responses.Majority of the identified proteins were down-regulated in both tissues after exposure to either AgNPs or AgNO3 ; in roots, the most affected proteins were those involved inresponse to abiotic and biotic stimuli and oxidative stress, while in leaf, both treatments had the most prominent effect on photosynthesis-related proteins. However, sinceAgNPs induced higher suppression of protein abundance than AgNO3, we conclude that AgNP effects can, at leastpartially, be attributed to nanoparticle form.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

Napomena
FP7-INFRASTRUCTURES-2012-1, Enabling Science and Technology through European Electron Microscopy - ESTEEM2 Grant Agreement n: 312483

POVEZANOST RADA