Naslov
Antioxidative system of bovine follicles regarding stage of estrous cycle and folliculogenesis

Autori
Aladrović, Jasna ; Beer Ljubić, Blanka ; Laškaj, Renata ; Vranković, Lana ; Lojkić, Martina

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
The Animal Biology / Vlizlo, Vasyl - Lavov : National academy of Agrarian science of Ukraine the Institute of Animal Biology, 2019, 7-10

Skup
19th Middle-European Buiatric Congress

Mjesto i datum
Lavov, Ukrajina, 22.05.2019. - 25.05.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
bovine follicular fluid, antioxidative system, luteal stage of estrous cycle, follicular stage of estrous cycle

Sažetak
Background. Oxidative stress is important for promoting the oocyte maturation and ovulation within the follicle. The aim of the study was to examine activities of the superoxide dismutase (SOD), glutathione peroxidase (GPx) and biological antioxidant potential (BAP) involved in protection against free oxygen radicals and concentration of reactive oxygen metabolites (ROMs) in bovine follicular fluid. Methods. Bovine ovaries were obtained from a slaughterhouse. The stage of estrous cycle (follicular or luteal) was identified. Follicular fluids (FF) were collected by puncture from three categories of follicles: small (≤5 mm), medium (6-10 mm) or large (>10 mm) from ovaries in both follicular and luteal stage of estruos cycle. Results. The results indicate a significantly higher activity of SOD in bovine FF from follicles in luteal stage of estruos cycle than in follicular stage (p=0.037). In FF of follicles in both stages of estrous cycle, SOD activity and BAP were significantly higher in FF of small and medium sized follicles than in large ones (p=0.000 both). The ROMs significantly declined from small to large follicles in FF collected during both, luteal and follicular phase of estrous cycle (p=0.005 both). Activity of GPx showed no significant differences regarding neither estrous cycle nor size of follicles. Conclusions. Results indicate similar antioxidative properties of bovine follicles during both luteal/follicular stage of estrous cycle with higher SOD activity in FF of follicles in luteal stage of estrous cycles which undergo atresia.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA