Pretraga

Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 1002539

Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma

Trbojević-Akmačić, Irena; Nemec, Blaž; Vidic, Urška; Malić, Suzana; Miklić, Karmela; Černigoj, Urh; Vidič, Jana; Lendero Krajnc, Nika; Štrancar, Aleš; Lauc, Gordan et al.
Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma // Croatica Chemica Acta, 89 (2016), 2; 203-211 doi:10.5562/cca2815 (recenziran, članak, znanstveni)

CROSBI ID: 1002539 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma

Autori
Trbojević-Akmačić, Irena ; Nemec, Blaž ; Vidic, Urška ; Malić, Suzana ; Miklić, Karmela ; Černigoj, Urh ; Vidič, Jana ; Lendero Krajnc, Nika ; Štrancar, Aleš ; Lauc, Gordan ; Lenac Roviš, Tihana ; Pučić-Baković, Maja

Izvornik
Croatica Chemica Acta (0011-1643) 89 (2016), 2; 203-211

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
high-throughput ; immunoaffinity chromatography ; monoliths ; N-glycosylation ; oriented antibody immobilization ; transferrin

Sažetak
Changes in protein glycosylation are related to different diseases and have a potential as diagnostic and prognostic disease biomarkers. Transferrin (Tf) glycosylation changes are common marker for congenital disorders of glycosylation. However, biological interindividual variability of Tf N-glycosylation and genes involved in glycosylation regulation are not known. Therefore, high-throughput Tf isolation method and large scale glycosylation studies are needed in order to address these questions. Due to their unique chromatographic properties, the use of chromatographic monoliths enables very fast analysis cycle, thus significantly increasing sample preparation throughput. Here, we are describing characterization of novel immunoaffinity-based monolithic columns in a 96- well plate format for specific high-throughput purification of human Tf from blood plasma. We optimized the isolation and glycan preparation procedure for subsequent ultra performance liquid chromatography (UPLC) analysis of Tf N- glycosylation and managed to increase the sensitivity for approximately three times compared to initial experimental conditions, with very good reproducibility.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Biotehnologija



POVEZANOST RADA

Projekti:
EK-FP7-324400 - Methods for high-throughput glycoproteomic analysis (HTP-GLYCOMET) (Jonjić, Stipan, EK - FP7-PEOPLE-2012-IAPP) ( CroRIS)
EK-FP7-278535 - Methods for high-throughput (HTP) analysis of protein glycosylation (HIGHGLYCAN) (Lauc, Gordan, EK - FP7-HEALTH-2011-two-stage) ( CroRIS)

Ustanove:
Medicinski fakultet, Rijeka,
GENOS d.o.o.,
Sveučilište u Rijeci

Profili:

Avatar Url Gordan Lauc (autor)

Avatar Url Tihana Lenac Roviš (autor)

Avatar Url Maja Pučić Baković (autor)

Avatar Url IRENA TRBOJEVIĆ AKMAČIĆ (autor)

Poveznice na cjeloviti tekst rada:

Pristup cjelovitom tekstu rada doi hrcak.srce.hr

Citiraj ovu publikaciju:

Trbojević-Akmačić, Irena; Nemec, Blaž; Vidic, Urška; Malić, Suzana; Miklić, Karmela; Černigoj, Urh; Vidič, Jana; Lendero Krajnc, Nika; Štrancar, Aleš; Lauc, Gordan et al.
Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma // Croatica Chemica Acta, 89 (2016), 2; 203-211 doi:10.5562/cca2815 (recenziran, članak, znanstveni)
Trbojević-Akmačić, I., Nemec, B., Vidic, U., Malić, S., Miklić, K., Černigoj, U., Vidič, J., Lendero Krajnc, N., Štrancar, A. & Lauc, G. (2016) Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma. Croatica Chemica Acta, 89 (2), 203-211 doi:10.5562/cca2815.
@article{article, author = {Trbojevi\'{c}-Akma\v{c}i\'{c}, Irena and Nemec, Bla\v{z} and Vidic, Ur\v{s}ka and Mali\'{c}, Suzana and Mikli\'{c}, Karmela and \v{C}ernigoj, Urh and Vidi\v{c}, Jana and Lendero Krajnc, Nika and \v{S}trancar, Ale\v{s} and Lauc, Gordan and Lenac Rovi\v{s}, Tihana and Pu\v{c}i\'{c}-Bakovi\'{c}, Maja}, year = {2016}, pages = {203-211}, DOI = {10.5562/cca2815}, keywords = {high-throughput, immunoaffinity chromatography, monoliths, N-glycosylation, oriented antibody immobilization, transferrin}, journal = {Croatica Chemica Acta}, doi = {10.5562/cca2815}, volume = {89}, number = {2}, issn = {0011-1643}, title = {Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma}, keyword = {high-throughput, immunoaffinity chromatography, monoliths, N-glycosylation, oriented antibody immobilization, transferrin} }
@article{article, author = {Trbojevi\'{c}-Akma\v{c}i\'{c}, Irena and Nemec, Bla\v{z} and Vidic, Ur\v{s}ka and Mali\'{c}, Suzana and Mikli\'{c}, Karmela and \v{C}ernigoj, Urh and Vidi\v{c}, Jana and Lendero Krajnc, Nika and \v{S}trancar, Ale\v{s} and Lauc, Gordan and Lenac Rovi\v{s}, Tihana and Pu\v{c}i\'{c}-Bakovi\'{c}, Maja}, year = {2016}, pages = {203-211}, DOI = {10.5562/cca2815}, keywords = {high-throughput, immunoaffinity chromatography, monoliths, N-glycosylation, oriented antibody immobilization, transferrin}, journal = {Croatica Chemica Acta}, doi = {10.5562/cca2815}, volume = {89}, number = {2}, issn = {0011-1643}, title = {Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma}, keyword = {high-throughput, immunoaffinity chromatography, monoliths, N-glycosylation, oriented antibody immobilization, transferrin} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus

Citati:

    Altmetrijski pokazatelji:


    Podijeli:





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font
    CROSBI koristi kolačiće (cookies) kako bi poboljšao funkcionalnost stranice.