Naslov
Chronic stress alters expression of insulin and leptin receptors in the brain of aged male rats

Autori
Majić, Ljiljana ; Balog, Marta ; Mikulec, Katarina ; Lulić, Doris ; Ivić, Vedrana ; Heffer, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
RECOOP 14th Bridges in Life Sciences and 2nd RECOOP-KFSD International Student Conference 2019 Abstract Book / Vari, Sandor G. - , 2019, 39-39

ISBN
978-615-6006-00-4

Skup
14th Bridges in Life Sciences Annual Scientific Meeting (RECOOP)

Mjesto i datum
Bratislava, Slovačka, 10.04.2019. - 14.04.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
chronic stress, aging, rat model, brain

Sažetak
Introduction: Chronic stress affects brain via HPA axis and changes glucose metabolism. Response to stress is gender and age specific - females endure stress better than males until menopause. Receptors involved in energy metabolism could contribute to stress response in the brain. Therefore, we analyzed insulin (IR) and leptin receptors (ObR) in hippocampi (HIP) and cerebella (CER) of stressed male and female rats to determine whether stress causes gender and age differences in neuronal metabolism. Methods: Male and female Sprague-Dawley rats were divided in young (6.5 months old) and old (14.5 months old) groups. Chronic stress protocol was performed in three 10-day stress sessions each separated by 2 weeks of no stress period. IR and ObR were detected in HIP and CER by immunohistochemistry and Western blot. All data was analyzed by the Statistica software. Results: Both immunohistochemistry and Western blot showed prominent decrease of IR expression in HIP and CER of old males upon stress compared to appropriate control group, what was exactly contrary to young males and females under stress. Immunohistochemistry of ObR had similar pattern: the expression was decreased in old stressed males in both tissues and it was increased in young stressed males. Western blot revealed long and short form of ObR which had more complex pattern of changes in males and females. The long form was expressed exactly opposite to the pattern revealed by immunohistochemistry. Discussion and Conclusion: Both, IR and ObR, are strongly influenced by stress in old males. Resistance of both receptors could be hypothesized in the case of old stressed males which could lead to impaired neuronal energy metabolism, central diabetes and neuronal death. Stress regulation in the brain is sex- and age-specific and old animals tend to show a stronger stress response than young animals what could lead to energy shortage and neurodegeneration. Acknowledgments: This study has been funded by Croatian Science Foundation (IP-2014-09-2324) and Cedars-Sinai Medical Center’s International Research and Innovation in Medicine Program and the RECOOP HST Association. Part of this study was performed at the Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, under the supervision of prof. Robert Gaspar. Ethical Committee Approval: Experiments were carried out at Animal Facility of Faculty of Medicine Osijek and was approved under class number 602-04/14-08/06 and registration number 2158-61-07-14-118 and Animal Facility of Faculty of Pharmacy, Szeged, approval number: CSI/01/3796-7/2015.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA