engleski

PD-L1 expression in various NSCLC cytology samples prepared as non-cell blocks

To compare PD-L1 expression between cytology samples obtained with bronchoscopy (bronchial washing/brushing ; BW/BB and transbronchial fine needle aspirations ; TBFNA) and other cytology samples such as pleural effusion, FNA of peripheral lymph nodes, FNA of skin nodules and transthoracic FNA/biopsy ; all prepared as non-cell block cytology. Materials and methods: We compared archive records of the result of PD-L1 expression in relation to specimen type (bronchoscopic and non-bronchoscopic samples) prepared as smears at our institution over a one year period. Air dried cytology smears and cytospins of NSCLC were stained with Anti-PD-L1, Clone 22C3, Dako on Autostainer. PD-L1 protein expression was scored by using Tumor Proportion Score (TPS) with positive cut-off of ≥1%. The slides were routinely examined and scored by two cytologists. Result: Out of 393 cytological smears that underwent PD-L1 scoring we have compared 341 smears. There were 216 samples of materials collected during bronchoscopy and 125 of various other samples ; 37 were obtained by FNA of peripheral lymph nodes, 28 by FNA of skin nodules, 28 by transthoracic FNA/biopsy and 32 pleural effusion. PD-L1 stained negative in total of 179/341 (52, 49%) samples. PD-L1 stained positive in total of 162/341(47, 50%) samples of which 105 were obtained with bronchoscopy and 57 of samples collected with other methods. Among of total PD-L1 positive smears, 72 were PD-L1 positive ≥ 50% ; 45/105 (42, 8%) were bronchoscopical samples and 27/57(47, 3%) other samples. Comparison in PD-L1 expression between two groups of samples showed no statistical significant difference among groups (X2 test, df 1, p=0, 591). Comparison between two groups of PD-L1 positive ≥ 50% showed no statistical significant difference among samples groups (X2 test, df 1, p=0, 581). Conclusion: There were no statistical significant differences in PD-L1 protein expression between samples collected during bronchoscopy and non-bronchoscopic samples prepared as non-cell block cytology. PD-L1 expression was independent of cytology sample type in our study.

PD-L1, NSCLC, Cytology

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