Aspergillus screening difficulties in pulmonary cytology samples (CROSBI ID 675962)
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Podaci o odgovornosti
Harabajsa, Suzana ; Markanović, Manda ; Pešut, Ana ; Vrabec Branica, Božica ; Šimić, Vesna ; Mareković, Ivana ; Smojver-Ježek, Silvana
engleski
Aspergillus screening difficulties in pulmonary cytology samples
Aim: To present Aspergillus screening difficulties in pulmonary cytology samples in correlation with microbiological fungal culture results. Methods: Aspergillus is the most common fungal organism followed by Candida in pulmonary cytology samples. The six year retrospective analysis included 27 cytology samples and corresponding cytological final reports of Aspergillus in Pulmonary Cytology Division. Microscopical findings in May Grünwald Giemsa slides (MGG) were uniform, septate, dichotomous, 45° branching (Y-shaped) hyphae consistent with Aspergillus. The cytological final reports were correlated with the microbiological fungal culture results. Results: Among 27 cytology samples with Aspergillus resembling morphology were 14 washing aspirates, six bronchial brushings, four imprint materials, two sputum samples and one transbronchial fine needle aspiration. Inflammation elements, poorly preserved hyphae and necrosis were also present in cytology samples. Cytology samples and microbiological fungal cultures were simultaneously obtained in 16 (16/27) cases. Fungal culture reports were positive for A. fumigatus, Aspergillus spp, A. flavus or A. niger in 14 (14/16) and negative in two (2/16) cases. In three (3/14) cases of Aspergillus positive fungal cultures and two (2/2) cases of Aspergillus negative fungal cultures Candida was also present. In six (6/27) cases with positive cytology samples subsequent fungal cultures were obtained. All six subsequent fungal cultures were negative for Aspergillus but one (1/6) of them had a prior history report of Aspergillus positive fungal culture and two (2/6) were positive for Candida albicans. In five cases cytological positive final reports were correlated with previous microbiological fungal culture results. Among previous fungal cultures, one (1/5) was positive for Aspergillus, one (1/5) for Candida albicans and three (3/5) were both negative. Conclusion: Morphological identification of Aspergillus in pulmonary cytology samples can be challenging. There is no morphological difference in cytological samples to differentiate between colonization and invasive infection. To determine the significance of cytological final reports of Aspergillus, microbiological fungal culture, fungal biomarkers, clinical and radiographic findings should be considered.
Aspergillus ; cytology ; microbiology ; fungal culture
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Podaci o prilogu
2019.
nije evidentirano
Podaci o matičnoj publikaciji
Podaci o skupu
Toraks 2019
poster
10.04.2019-13.04.2019
Zagreb, Hrvatska