engleski

Behavior of algal cells under stress in laboratory conditions: Electrochemical approach

Marine microalgae play a key role in global primary production, the main biogeochemical cycles and present the base of the food web in the aquatic environment. Algae cell response is a reliable indicator of changes in ecosystems because of its short life cycle responds quickly to changes in the environment. The aim of the study which is part of the graduate Aim is to link the algal cell behavior (in terms of their adhesion at the model interface [1], and physiological activity) to the stressors (salinity, the presence of heavy metal of cadmium) for better understanding of survival strategy and algal cell adaptation in aquatic systems. The selected algal monoculture system is the Dunaliella tertiolecta that has only a cell membrane, pronounced motility and tolerates a wide range of salinity. Optical microscopy is used to visualize cellular shape, and to determine cell abundance. The electrochemical method of amperometry at the dropping mercury electrode is used to characterize cell suspensions before and after exposure to stress and therefore results will be presented in terms of concentration, level of polydispersity and adhesion behavior of cells at the wide potential range. Acknowledgment: This study is supported by the International Visegrad Fund Grant No 21720055 "Algal cell biophysical properties as markers for environmental stress in aquatic systems" N. Ivošević DeNardis (PI). [1] N. Ivošević DeNardis, J. Pečar Ilić, I. Ružić, G. Pletikapić, Electrochim. Acta, 176 (2015) 743-754.

adhesion ; algal cells ; amperometry ; cadmium ; physiological activity ; stress

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