engleski

Fish photobacteriosis – the importance of rapid and accurate identification of Photobacterium damselae subsp. piscicida

MALDI-TOF MS was tested for identification of Photobacterium damselae subsp. piscicida on isolates grown on two media, cultured at three incubation times, applied on the target plate by the direct sample spotting (DS), the on- target extraction (OTE) and by the full extraction (FE) method, in triplicates. Identification of samples grown on blood agar (BA) outperformed identification on tryptic soy agar (TSA) by 0.64% for DS and OTE. The OTE gave highest scores in both culture media, all incubation times and replicates. Reliable 24 h species identification were 61.54 %, 84.61 %, and 53.85 % for samples grown on TSA and identified by DS, OTE and FE, respectively. For isolates grown on BA they were 76.92 %, 96.15 %, and 30.77 %, respectively. When identified by OTE, the 48 h identification was 93.58 %, but for 72 h declined to 71.79 %. The reliable identification with the highest score from the first measurement was 100 % only for OTE from BA (24 h), whereas OTE from TSA gave 84.61 % (24 h), 76.92 % (48 h), and 84.61 % (72 h). The reliable MALDI-TOF MS identification of Ph. damselae subsp. piscicida is incubation time, media, target plate preparation, and replicate dependent.

Photobacterium damselae subsp. piscicida ; MALDI-TOF MS

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