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Pregled bibliografske jedinice broj: 997807

Concomitant resistance to paclitaxel in an ovarian cancer cell variant selected with carboplatin


Kralj, Juran; Duran, George E.; Stupin Polančec, Darija; Bačić, Niko; Sikic, Branimir I.; Brozovic, Anamaria
Concomitant resistance to paclitaxel in an ovarian cancer cell variant selected with carboplatin // 4th Congress of Croatian Geneticists with international participation : book of abstracts / Šarčević, Hrvoje ; Ugarković, Đurđica ; Vujaklija, Dušica ; Svetec, Ivan Krešimir ; Svetec Miklenić, Marina. (ur.).
Zagreb: Hrvatsko genetičko društvo, 2018. str. 51-51 (poster, domaća recenzija, sažetak, znanstveni)


CROSBI ID: 997807 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Concomitant resistance to paclitaxel in an ovarian cancer cell variant selected with carboplatin

Autori
Kralj, Juran ; Duran, George E. ; Stupin Polančec, Darija ; Bačić, Niko ; Sikic, Branimir I. ; Brozovic, Anamaria

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
4th Congress of Croatian Geneticists with international participation : book of abstracts / Šarčević, Hrvoje ; Ugarković, Đurđica ; Vujaklija, Dušica ; Svetec, Ivan Krešimir ; Svetec Miklenić, Marina. - Zagreb : Hrvatsko genetičko društvo, 2018, 51-51

ISBN
978-953-57128-1-7

Skup
4th Congress of Croatian Geneticists with international participation

Mjesto i datum
Krk, Hrvatska, 26-29.09.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
drug resistance ; ovarian cancer ; tubulin ; molecular mechanisms ; therapy

Sažetak
Most epithelial ovarian cancer patients are diagnosed with advanced-stage disease due to the late appearance of symptoms and lack of early diagnostic methods/markers. The major problem for successful therapy is the development of tumour drug resistance during carcinogenesis (20-30%) and upon exposure to chemotherapy. The ovarian cancer cell line OVCAR-3/CBP was established by treatment of the ovarian adenocarcinoma cell line OVCAR-3 with long-term, stepwise selection in carboplatin (CBP) up to 25 μM. The variant is ~1.5-2-fold resistant to CBP, with cross-resistance to paclitaxel (TAX, ~4-fold), and presents with a mesenchymal-like phenotype. The increased expression of NHE-1, ATP7-B and decreased expression of ABCC2 and CTR-1 implied decreased total cell platination as a possible mode of CBP resistance, which was confirmed by flame atomic absorption spectrometry. Despite the increased level of ABCB1 transcripts, OVCAR-3/CBP did not efflux [3H]-docetaxel differently compared to parental cells, and the P-glycoprotein inhibitor PSC-833 did not alter these drug accumulation profiles. This indicates that the TAX resistance in OVCAR-3/CBP is non-MDR, but is associated with elevated TUBB3 (class III beta-tubulin) content along with total α- and β-tubulin relative to parental OVCAR-3 cells. In summary, drug selection with carboplatin in an ovarian cancer cell line resulted in non-MDR cross-resistance to paclitaxel. Experiments investigating the functional significance of altered tubulin content and microtubule dynamicity in response to drug exposure in OVCAR-3/CBP are on-going.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
HRZZ-IP-2016-06-1036 - Određivanje ključnih molekula epitelno-mezenhimalne tranzicije kao mogućih ciljeva za terapiju raka jajnika (DEvOuT) (Brozović, Anamaria, HRZZ - 2016-06) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb,
Fidelta d.o.o.

Poveznice na cjeloviti tekst rada:

pubweb.carnet.hr

Citiraj ovu publikaciju:

Kralj, Juran; Duran, George E.; Stupin Polančec, Darija; Bačić, Niko; Sikic, Branimir I.; Brozovic, Anamaria
Concomitant resistance to paclitaxel in an ovarian cancer cell variant selected with carboplatin // 4th Congress of Croatian Geneticists with international participation : book of abstracts / Šarčević, Hrvoje ; Ugarković, Đurđica ; Vujaklija, Dušica ; Svetec, Ivan Krešimir ; Svetec Miklenić, Marina. (ur.).
Zagreb: Hrvatsko genetičko društvo, 2018. str. 51-51 (poster, domaća recenzija, sažetak, znanstveni)
Kralj, J., Duran, G., Stupin Polančec, D., Bačić, N., Sikic, B. & Brozovic, A. (2018) Concomitant resistance to paclitaxel in an ovarian cancer cell variant selected with carboplatin. U: Šarčević, H., Ugarković, Đ., Vujaklija, D., Svetec, I. & Svetec Miklenić, M. (ur.)4th Congress of Croatian Geneticists with international participation : book of abstracts.
@article{article, author = {Kralj, Juran and Duran, George E. and Stupin Polan\v{c}ec, Darija and Ba\v{c}i\'{c}, Niko and Sikic, Branimir I. and Brozovic, Anamaria}, year = {2018}, pages = {51-51}, keywords = {drug resistance, ovarian cancer, tubulin, molecular mechanisms, therapy}, isbn = {978-953-57128-1-7}, title = {Concomitant resistance to paclitaxel in an ovarian cancer cell variant selected with carboplatin}, keyword = {drug resistance, ovarian cancer, tubulin, molecular mechanisms, therapy}, publisher = {Hrvatsko geneti\v{c}ko dru\v{s}tvo}, publisherplace = {Krk, Hrvatska} }
@article{article, author = {Kralj, Juran and Duran, George E. and Stupin Polan\v{c}ec, Darija and Ba\v{c}i\'{c}, Niko and Sikic, Branimir I. and Brozovic, Anamaria}, year = {2018}, pages = {51-51}, keywords = {drug resistance, ovarian cancer, tubulin, molecular mechanisms, therapy}, isbn = {978-953-57128-1-7}, title = {Concomitant resistance to paclitaxel in an ovarian cancer cell variant selected with carboplatin}, keyword = {drug resistance, ovarian cancer, tubulin, molecular mechanisms, therapy}, publisher = {Hrvatsko geneti\v{c}ko dru\v{s}tvo}, publisherplace = {Krk, Hrvatska} }




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