How B-6 helps B-12: The roles of B-6, B-12, and the enzymes in aminomutase-catalyzed reactions (CROSBI ID 263848)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Wetmore, Stacey D. ; Smith, David M. ; Radom, Leo
engleski
How B-6 helps B-12: The roles of B-6, B-12, and the enzymes in aminomutase-catalyzed reactions
The 1, 2-shift of an amino group appears to be a demanding task. Our calculations show that the rearrangement may be efficiently accomplished as a result of an intricate relationship between the enzyme and its cofactors. Thus, we believe that coenzyme B12 is responsible for activating the substrate by removal of a hydrogen atom. Vitamin B6 (PLP) introduces a seemingly essential double bond into the migrating group, as well as imparting the potential for the reaction intermediate to be captodatively stabilized. The enzyme itself holds all the components in place and provides an environment in which the pyridine nitrogen can be fully (or partially) protonated. This cooperative action of the enzyme and cofactors is able to mediate an otherwise difficult reaction.
METHYLMALONYL-COA MUTASE ; PARTIAL-PROTON-TRANSFER ; MECHANISM ; ADENOSYLCOBALAMIN ; REARRANGEMENT
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Podaci o izdanju
122 (41)
2000.
10208-10209
objavljeno
0002-7863
10.1021/ja001651y