engleski

GENE POLYMORPHISMS OF DNA METHYLTRANSFERASE 3B IN WOMEN WITH SPONTANEOUS PRETERM BIRTH

Aim: The aim of this study was to evaluate the potential association between spontaneous or idiopathic PTB (SPTB) and DNMT3B gene polymorphisms in Slavic women, and their contribution to clinical characteristics of women with SPTB and their new-borns (family history of PTB, maternal smoking before pregnancy, maternal age, gestational week at delivery and fetal birth weight). Patients and methods: A total of 162 women with SPTB and 162 women with term delivery were included in a case-control study. Genotyping of DNMT3B rs1569686 and DNMT3B rs2424913 single nucleotide polymorphisms was performed using polymerase chain reaction and restriction fragment length polymorphism methods. Results: DNMT3B rs1569686 and rs2424913 minor alleles (T) were significantly more frequent in women with familial PTB than non-familial PTB, and contributed to a 3.30 and 3.54 increased odds for familial PTB under dominant genetic models (95 % CI = 1.53-7.14, P = 0.003 and 95 % CI = 1.56-8.01, P = 0.002). Furthermore, the DNMT3B rs1569686 and rs2424913 T alleles were significantly more frequent in women with SPTB who smoked before pregnancy, reaching the most significant association under additive genetic models (OR = 6.86, 95 % CI = 2.25-20.86, P ˂ 0.001 and OR = 3.77, 95 % CI = 1.36-10.52, P = 0.011). Conclusion: The DNMT3B rs1569686 and rs2424913 gene polymorphisms in Slavic women might be associated with a positive family history of PTB and smoking status before pregnancy.

DNA methyltransferases ; Pregnancy ; Preterm birth ; Single nucleotide polymorphism

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