Pregled bibliografske jedinice broj: 997244
Angiotensin II Supplementation by Miniosmotic Pumps Reduces the Level of Oxidative Stress Induced by High Dietary Salt Intake in Rat Microcirculation
Angiotensin II Supplementation by Miniosmotic Pumps Reduces the Level of Oxidative Stress Induced by High Dietary Salt Intake in Rat Microcirculation // Journal of Vascular Research 56:1–134
Maastricht, Nizozemska, 2019. str. 78-79 doi:10.1159/000499516 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 997244 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Angiotensin II Supplementation by Miniosmotic Pumps Reduces the Level of Oxidative Stress Induced by High Dietary Salt Intake in Rat Microcirculation
Autori
Matić, Anita ; Mihaljević, Zrinka ; Jukić, Ivana ; Kolobarić, Nikolina ; Šušnjara, Petar ; Stupin, Ana ; Drenjančević, Ines
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Journal of Vascular Research 56:1–134
/ - , 2019, 78-79
Skup
ESM-EVBO 2019: 3rd Joint Meeting of the European Society for Microcirculation (ESM) and the European Vascular Biology Organization (EVBO)
Mjesto i datum
Maastricht, Nizozemska, 15-18.04.2019
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
high salt diet ; oxidative stress ; angiotensin II ; Sprague-Dawley rats
Sažetak
Introduction: Previously, we demonstrated that suppressed levels of angiotensin II by high salt diet (HS) impaired endothelium-dependent dilation and reduced nitric oxide (NO) bioavailability due to increased endothelial levels of reactive oxygen species (ROS) in middle cerebral arteries (MCAs) of rats (J Physiol 2016 ; 594(17):4917–31 ; AJP 2018 ; 315(3):H718– H730). Present study aimed to determine the effect of angiotensin II supplementation on vascular NO and superoxide/ROS production and vascular antioxidative enzymes’ mRNA expression. Methods: 9–11 weeks old healthy male Sprague- Dawley rats were randomly assigned to 3 groups (n = 6–8 rats/group): low saltdiet group (LS group, 0.4% NaCl in rat chow) ; HS group (4% NaCl in rat chow for 1 week) and HS+angiotensin II group (HS diet for 7 days, additionally 4th-7th day subpressor doses of angiotensin II via osmotic minipump (100 ng/kg/min/3 days). Following dietary protocol, rats were anesthetized with ketamine (75 mg/kg) and midazolam (2.5 mg/kg) and sacrificed by decapitation. MCA were isolated and cannulated on pressure myograph, under flow (Δ80 mm Hg) or no flow conditions. Endothelial NO and superoxide/ ROS production (determined by the nonfluorescent 4, 5-diaminofluorescein and with dihydroethidine, respectively) were measured by direct flourescence microscopy. In a separate group of experiments, all surface brain blood vessels (BBVs) were isolated and collected for rtPCR expression measurements of mRNA of Cu/Zn SOD, MnSOD, EC-SOD, GPx1 and GPx4 and catalase (CAT). All experimental procedures were approved by the local Ethical Committee and conformed to the EU Directive 86/609. Data were analyzed using One-Way ANOVA, presented as mean±SD. p < 0.05 was considered significant. Results: Basal NO production in no-flow condition was similar among groups. L-NAME blocked the production of NO in each group (basal vs. L-NAME LS:35.4 ± 7.09 vs. 22.8 ± 8.01 ; HS: 32.4 ± 14.5 vs. 14.2 ± 2.58 ; HS+ANG II 35.22 ± 4.67 vs. 12.23 ± 3.27). Flow-induced NO production was significantly lower in HS group (21.34 ± 1.37) compared to LS (31.26 ± 7.20) and HS+angiotensin II (40.80 ± 8.22) groups. L-NAME blocked flow- induced NO production similarly among groups (p > 0.05). No-flow superoxide/ ROS levels in the BBVs endothelium were not significantly different among the groups (p > 0.05). However, flow increased level of superoxide/ROS in the HS group (9.16 ± 1.52) compared with the other groups (LS 7.30 ± 1.13 and HS+angiotensin II 6.44 ± 1.56). There was significantly higher expression of only of EC-SOD and GPx4 in HS+angiotensin II (EC-SOD 1.75 ± 0.43, GPx4 2.15 ± 0.41) group compared to LS (EC-SOD 0.60 ± 0.20, GPx4 0.82 ± 0.17) and HS group (EC-SOD 0.51 ± 0.11, GPx4 0.64 ± 0.07). Conclusions: Suppressor doses of angiotensin II restored NO production by decreasing flow-induced superoxide/ROS production in MCAs, due to increased antioxidant capacity of vasculature.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
HRZZ-IP-2014-09-6380 - poremećena vazorelaksacija i endotelno-leukocitna interakcija (ELI) u razvoju aterosklerotskih lezija (V-ELI Athero) (Drenjančević, Ines, HRZZ - 2014-09) ( POIROT)
Ustanove:
Medicinski fakultet, Osijek,
Fakultet za dentalnu medicinu i zdravstvo, Osijek
Profili:
Anita Matić
(autor)
Ana Stupin
(autor)
Zrinka Mihaljević
(autor)
Ivana Jukić
(autor)
Ines Drenjančević
(autor)
Citiraj ovu publikaciju
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE