engleski

A collagen 2 reporter for online identification of chondrogenic microenvironments

Cell-based tissue engineering is a promising method for repair of injured cartilage, however, the outcome depends highly on the continued expression of cartilage markers by the transplanted cells. Collagen 2 is a main structural component of cartilage and hence a method to monitor its expression under changing experimental microenvironments would be of great value. In this study, the human collagen II promoter was cloned into a lentiviral vector and used to drive the expression of green fluorescent protein (GFP) in human chondroprogenitor cells. The resulting promoter reporter gene construct provides the opportunity for non-invasive and repeated imaging of chondrocyte differentiation.

cartilage ; collagen 2

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