engleski

Histomorphometric analysis of human subcutaneous and visceral adipose tissue samples collected from women undergoing elective laparoscopic cholecystectomy

Introduction: In cases of chronic nutritional surplus, adipose tissue enlarges through processes of cellular hypertrophy and/or hyperplasia. If this state persists, homeostatic machenisms fail and pathological expansion with microinflammation and subsequent tissue dysfunction occurs. This has been extensively studied in animal models, however, not enough data was published on whether similar process of tissue remodeling takes place in humans. Methods: A sample of subcutaneous and visceral fat tissue, from abdominal wall and perihaepatal pad respectively, was collected from each of 25 women (age 36±7.19, min. 22, max. 44 years old) during an elective laparoscopic cholecystectomy at the University Hospital Osijek. Extensive exclusion criteria on patient selection were applied (comorbidities, oral contraceptives, menstrual cycle, inflammation, acute cholecystitis etc.) The tissue samples were paraffin-embeded, hystologically stained, photographed and subsequently histomorphometrically analysed in CellProfiler (version 3.1.5). Additional anthropological measures were obtained from the subjects, including Body mass index (BMI) and Hip to waist ratio. Results: Median adipocyte surface area (MASA) in visceral compartment...

Adipose Tissue ; Obesity ; Metabolic Syndrome

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