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Pregled bibliografske jedinice broj: 995419

Is Sample Mixing Necessary Prior to HbA1c Measurement on the BioRad D-10 Analyser?


Brkić, Ivona; Kranjčina, Ariana; Starčić, Jelena; Žarak, Marko; Škorvaga, Sanja; Stančin, Nevenka; Živković, Marcela
Is Sample Mixing Necessary Prior to HbA1c Measurement on the BioRad D-10 Analyser? // 5th EFLM Conference on Preanalytical Phase Zagreb (HR), 22-23 March 2019
Zagre, Hrvatska, 2019. str. 69-69 doi:10.1515/cclm-2019-0104 (poster, međunarodna recenzija, sažetak, stručni)


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Naslov
Is Sample Mixing Necessary Prior to HbA1c Measurement on the BioRad D-10 Analyser?

Autori
Brkić, Ivona ; Kranjčina, Ariana ; Starčić, Jelena ; Žarak, Marko ; Škorvaga, Sanja ; Stančin, Nevenka ; Živković, Marcela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
5th EFLM Conference on Preanalytical Phase Zagreb (HR), 22-23 March 2019 / - , 2019, 69-69

Skup
5th EFLM Conference on Preanalytical Phase

Mjesto i datum
Zagre, Hrvatska, 22-23.03.20198

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
HbA1c measurement, diabetes mellitus, sample mixing, HPLC

Sažetak
BACKGROUND-AIM: HbA1c values are an important indicator of patient’s glycemic control and diagnosis of diabetes mellitus. Our HPLC analyzer BioRad D-10 measures HbA1c in whole blood, a maximum of 10 samples in series. Aim of this study was to determine whether sample mixing affects HbA1c measurement, since the analyzer does not mix the samples and the analysis of a full series takes 30 minutes. METHODS: HbA1c values of 30 samples (K3-EDTA anticoagulant, BD Vacutainer) were mixed and measured routinely (reference measure- ment, R) on the analyzer BioRad D-10 (BioRad Laboratories, Hercules, USA). HbA1c was then measured after 30 and 60 minutes (first, M1, and second, M2 measurement) of sedimentation, without mixing prior to analysis. The data was tested for normality with Kolmogorov-Smirnov test and differences were tested with t-test for paired samples. RESULTS: Measured values of HbA1c ranged from 4, 3% to 11, 2%. All three measurement groups were normally distributed, with arithmetic means and standard deviations as follows, R: 6, 3% ± 1, 2% ; M1: 6, 2% ± 1, 2% and M2: 6, 2% ± 1, 2%. Obtained P-values were found statistically significant between the reference and first measurement (P = 0, 009) and reference and second measurement (P < 0, 001). Also, since M1 and M2 measurements are compared to the initial, reference measurement, relative bias values were calculated between R and M1 and R and M2. The mean bias values were 1, 5% for R and M1, and 1, 3% for R and M2 measurements. CONCLUSIONS: There was a statistically significant difference between the reference measurement and the measurements without sample mixing. However, obtained bias values are within the desirable biological variation criteria for bias (1, 5%). In conclusion, sample mixing prior to HbA1c measurement on the BioRad D-10 analyzer is necessary to obtain an accurate and clinically relevant HbA1c value.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti



POVEZANOST RADA


Ustanove:
Klinička bolnica "Dubrava"

Profili:

Avatar Url Nevenka Stančin (autor)

Avatar Url Marcela Živković (autor)

Avatar Url Marko Žarak (autor)

Poveznice na cjeloviti tekst rada:

Pristup cjelovitom tekstu rada doi www.degruyter.com

Citiraj ovu publikaciju:

Brkić, Ivona; Kranjčina, Ariana; Starčić, Jelena; Žarak, Marko; Škorvaga, Sanja; Stančin, Nevenka; Živković, Marcela
Is Sample Mixing Necessary Prior to HbA1c Measurement on the BioRad D-10 Analyser? // 5th EFLM Conference on Preanalytical Phase Zagreb (HR), 22-23 March 2019
Zagre, Hrvatska, 2019. str. 69-69 doi:10.1515/cclm-2019-0104 (poster, međunarodna recenzija, sažetak, stručni)
Brkić, I., Kranjčina, A., Starčić, J., Žarak, M., Škorvaga, S., Stančin, N. & Živković, M. (2019) Is Sample Mixing Necessary Prior to HbA1c Measurement on the BioRad D-10 Analyser?. U: 5th EFLM Conference on Preanalytical Phase Zagreb (HR), 22-23 March 2019 doi:10.1515/cclm-2019-0104.
@article{article, author = {Brki\'{c}, Ivona and Kranj\v{c}ina, Ariana and Star\v{c}i\'{c}, Jelena and \v{Z}arak, Marko and \v{S}korvaga, Sanja and Stan\v{c}in, Nevenka and \v{Z}ivkovi\'{c}, Marcela}, year = {2019}, pages = {69-69}, DOI = {10.1515/cclm-2019-0104}, keywords = {HbA1c measurement, diabetes mellitus, sample mixing, HPLC}, doi = {10.1515/cclm-2019-0104}, title = {Is Sample Mixing Necessary Prior to HbA1c Measurement on the BioRad D-10 Analyser?}, keyword = {HbA1c measurement, diabetes mellitus, sample mixing, HPLC}, publisherplace = {Zagre, Hrvatska} }
@article{article, author = {Brki\'{c}, Ivona and Kranj\v{c}ina, Ariana and Star\v{c}i\'{c}, Jelena and \v{Z}arak, Marko and \v{S}korvaga, Sanja and Stan\v{c}in, Nevenka and \v{Z}ivkovi\'{c}, Marcela}, year = {2019}, pages = {69-69}, DOI = {10.1515/cclm-2019-0104}, keywords = {HbA1c measurement, diabetes mellitus, sample mixing, HPLC}, doi = {10.1515/cclm-2019-0104}, title = {Is Sample Mixing Necessary Prior to HbA1c Measurement on the BioRad D-10 Analyser?}, keyword = {HbA1c measurement, diabetes mellitus, sample mixing, HPLC}, publisherplace = {Zagre, Hrvatska} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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