engleski

Effects of 1800 MHz non-thermal radiation on oxidative stress parameters in fibroblast cells

The aim of this study was to investigate possible connection between non-thermal radiofrequency exposure (RF) and development of oxidative stress. Fibroblast cells (V79) were exposed to 1800 MHz GSM modulated RF radiation for 10, 30 and 60 minutes inside of the Gigahertz Transverse Electromagnetic Cell (GTEM). The electric field strength was 30 V/m and specific absorption rate (SAR) was calculated to be 1.6 W/kg. Cell viability was determined by CCK-8 colorimetric assay and level of reactive oxygen species (ROS) was detected by fluorescent dyes dihydroethidium and 2′, 7′-dichlorodihydrofluorescein diacetate (DCFH-DA). Cellular antioxidant status was evaluated by measuring the level of reduced glutathione (GSH) and glutathione peroxidase activity, while protein and lipid oxidative damage was detected measuring concentrations of protein carbonyls and malondialdehyde (MDA). The viability of irradiated cells remained within normal physiological values regardless of exposure time. Higher level of ROS was observed after 10 or 60 minute irradiation, while greater GSH level was observed after 10-minute exposure. The activity of GSH-Px in exposed cell samples was higher than control, although it was not considered statistically significant. The applied radiation did not cause protein or lipid oxidative damage. The observed changes in the level of ROS and cellular antioxidant defence indicate transient oxidation-reduction imbalance in fibroblast cells following adaptation to the applied short-term RF exposure.

radiofrequency radiation ; reactive oxygen species ; antioxidan defence ; oxidative stress

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