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Editing the epigenome using a comprehensive CRISPR/Cas9-based molecular toolbox (CROSBI ID 674679)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Vojta, Aleksandar ; Josipović, Goran ; Tadić, Vanja ; Zoldoš, Vlatka Editing the epigenome using a comprehensive CRISPR/Cas9-based molecular toolbox. 2018. str. 47-47

Podaci o odgovornosti

Vojta, Aleksandar ; Josipović, Goran ; Tadić, Vanja ; Zoldoš, Vlatka

engleski

Editing the epigenome using a comprehensive CRISPR/Cas9-based molecular toolbox

The advent of the CRISPR/Cas9 technology revolutionized the field of genome engineering by providing a flexible platform for targeting almost any genomic region. Soon, fusions of the targeting moiety Cas9 with various effector domains enabled the construction of a novel class of molecular tools useful for gene regulation and epigenetic modifications. We focused on targeted CpG methylation and demethylation of gene promoters, which is a major epigenetic mechanism controlling gene expression. First, we constructed a modular molecular toolbox for introducing targeted epigenetic modifications, which enabled easy deployment and application to biological problems. After the challenging step of creating active fusions between Cas9 and DNMT3A or TET1 catalytic domains for CpG methylation/demethylation and careful characterization of the new molecular tools, we approached biological problems in the field of epigenetic regulation of protein glycosylation with special emphasis on the role of IgG glycosylation in inflammatory diseases. We are using this platform to reveal the underlying epigenetics responsible for the inflammatory phenotype common to many diseases with the aim of discovering robust biomarkers and also to pave the way for personalized and precise epigenetic therapy in the future.

CRISPR ; Cas9 ; epigenetics ; gene regulation

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Podaci o prilogu

47-47.

2018.

objavljeno

Podaci o matičnoj publikaciji

Podaci o skupu

5th Central-Eastern European Congress on Cell-Free Nucleic Acids

pozvano predavanje

21.06.2018-22.06.2018

Debrecen, Mađarska

Povezanost rada

Biologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)