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Pregled bibliografske jedinice broj: 994784

Editing the epigenome using a comprehensive CRISPR/Cas9-based molecular toolbox


Vojta, Aleksandar; Josipović, Goran; Tadić, Vanja; Zoldoš, Vlatka
Editing the epigenome using a comprehensive CRISPR/Cas9-based molecular toolbox // 5th Central-Eastern European Congress on Cell-Free Nucleic Acids
Debrecen, Mađarska, 2018. str. 47-47 (pozvano predavanje, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 994784 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Editing the epigenome using a comprehensive CRISPR/Cas9-based molecular toolbox

Autori
Vojta, Aleksandar ; Josipović, Goran ; Tadić, Vanja ; Zoldoš, Vlatka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
5th Central-Eastern European Congress on Cell-Free Nucleic Acids

Mjesto i datum
Debrecen, Mađarska, 21-22.06.2018

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
CRISPR ; Cas9 ; epigenetics ; gene regulation

Sažetak
The advent of the CRISPR/Cas9 technology revolutionized the field of genome engineering by providing a flexible platform for targeting almost any genomic region. Soon, fusions of the targeting moiety Cas9 with various effector domains enabled the construction of a novel class of molecular tools useful for gene regulation and epigenetic modifications. We focused on targeted CpG methylation and demethylation of gene promoters, which is a major epigenetic mechanism controlling gene expression. First, we constructed a modular molecular toolbox for introducing targeted epigenetic modifications, which enabled easy deployment and application to biological problems. After the challenging step of creating active fusions between Cas9 and DNMT3A or TET1 catalytic domains for CpG methylation/demethylation and careful characterization of the new molecular tools, we approached biological problems in the field of epigenetic regulation of protein glycosylation with special emphasis on the role of IgG glycosylation in inflammatory diseases. We are using this platform to reveal the underlying epigenetics responsible for the inflammatory phenotype common to many diseases with the aim of discovering robust biomarkers and also to pave the way for personalized and precise epigenetic therapy in the future.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA


Ustanove:
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Vlatka Zoldoš (autor)

Avatar Url Vanja Tadić (autor)

Avatar Url Goran Josipović (autor)

Avatar Url Aleksandar Vojta (autor)


Citiraj ovu publikaciju:

Vojta, Aleksandar; Josipović, Goran; Tadić, Vanja; Zoldoš, Vlatka
Editing the epigenome using a comprehensive CRISPR/Cas9-based molecular toolbox // 5th Central-Eastern European Congress on Cell-Free Nucleic Acids
Debrecen, Mađarska, 2018. str. 47-47 (pozvano predavanje, međunarodna recenzija, sažetak, znanstveni)
Vojta, A., Josipović, G., Tadić, V. & Zoldoš, V. (2018) Editing the epigenome using a comprehensive CRISPR/Cas9-based molecular toolbox. U: 5th Central-Eastern European Congress on Cell-Free Nucleic Acids.
@article{article, author = {Vojta, Aleksandar and Josipovi\'{c}, Goran and Tadi\'{c}, Vanja and Zoldo\v{s}, Vlatka}, year = {2018}, pages = {47-47}, keywords = {CRISPR, Cas9, epigenetics, gene regulation}, title = {Editing the epigenome using a comprehensive CRISPR/Cas9-based molecular toolbox}, keyword = {CRISPR, Cas9, epigenetics, gene regulation}, publisherplace = {Debrecen, Ma\djarska} }
@article{article, author = {Vojta, Aleksandar and Josipovi\'{c}, Goran and Tadi\'{c}, Vanja and Zoldo\v{s}, Vlatka}, year = {2018}, pages = {47-47}, keywords = {CRISPR, Cas9, epigenetics, gene regulation}, title = {Editing the epigenome using a comprehensive CRISPR/Cas9-based molecular toolbox}, keyword = {CRISPR, Cas9, epigenetics, gene regulation}, publisherplace = {Debrecen, Ma\djarska} }




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