engleski

The conservation status of Dalmatian pramenka sheep using high-throughput molecular information

The Dalmatian Pramenka (DAL) is the largest autochthonous population of sheep in Croatia that belongs to the long tailed Pramenka type of sheep, which are widespread over South-East Europe. We genotyped 32 DAL individuals on the Illumina Ovine SNP50 K BeadChip, and calculated parameters that are important for the conservation status: observed genome-wide heterozygosity (oHet), effective population size estimated via linkage (NeLD), and gametic (NeGD), disequilibrium and the ROH-based inbreeding level (FROH≤50g and FROH≤10g). A comparison with 24 Appenninica, 24 Arawapa, 24 Australian Merino, 24 Churra, 24 Finnsheep, 21 Merino Landschaf, 24 Massese and 24 Sardinian White revealed that DAL has the highest observed heterozygosity (oHet=0.375) and the second highest NeGD (124), as well as the lowest ROH- based inbreeding levels (FROH≤50g=0.021 and FROH≤10g=0.009). Interestingly, DAL has the highest estimated decline in NeLD (ΔNeLD=13), resulting in an extremely low NeLD of 15-31 (95% CI). Most likely, this notable difference is the consequence of the population growth of DAL in last generations, in contrast to the population decline in the period considered in historical NeLD estimates (12 to 100 generations back).

linkage effective population size ; gametic disequilibrium effective population size ; genomic inbreeding

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