engleski

Influence of hemolysis/lipemia on resistin and MPO ELISA tests

BACKGROUND-AIM. Hemolytic and lipemic samples in research ELISA tests can cause false low/high results. Peptide hormone resistin (RES) is potential marker of metabolic syndrome. Myeloperoxidase (MPO) is glycoprotein related to disorders like multiple sclerosis and atherosclerosis. The aim of our study was to investigate influence of hemolysis and lipemia on determination of RES and MPO using Human Resistin ELISA and Human MPO ELISA (BioVendor, Brno, Czech Republic). METHODS: Lithium heparin plasma sample was mixed with Lipofundin emulsion for testing of lipemia interference. Hemolysis was achieved by drawing aliquots of heparinized blood through 26 gauge needle. Index of hemolysis (H), lipemia (L) and triglyceride concentrations were measured on Architect (Abbott Laboratories, Abbott Park, Illinois, USA). Hemoglobin concentration was measured with hematology analyzer Sysmex XN-1000 (Sysmex, Kobe, Japan). Concentrations of RES and MPO were determined with Human Resistin ELISA and Human MPO ELISA test kits. All measurements were performed in triplicate. RESULTS: Lipemic plasma samples ranged from L index of -0.01 to 17.69 and triglyceride concentrations from 0.57 to 50.23 mmol/L. Hemolytic samples ranged from H index of 0.05 to 8.77 or from 0 to 8 g/L hemoglobin. RES concentration had 66% bias from initial concentration already at 1.34 H index or 1 g/L of hemoglobin. Bias of -7.9% was recorded from initial concentration at L index of 0.59 and triglyceride concentration of 2.60 mmol/L. MPO concentrations showed significant bias (58.7%) at the same hemoglobin concentration, while 33.8% bias was recorded for 1.28 L index or triglyceride concentration of 4.66 mmol/L. CONCLUSION: Interference testing for hemolysis and lipemia on ELISA tests for RES and MPO showed significant biases. Erroneus research results caused by such interferences contribute to misleading conclusions.

hemolysis, lipemia, ELISA tests, MPO, resistin

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