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Pregled bibliografske jedinice broj: 992449

Hyperbaric oxygenation affects acetylcholine- induced relaxation in female diabetic rats


(Institute of Physiology, Faculty of Medicine University of Ljubljana, Ljubljana, Slovenia) Manojlović, Dragan; Stupin, Ana; Mihaljević, Zrinka; Matić, Anita; Lenasi, Helena; Drenjančević, Ines
Hyperbaric oxygenation affects acetylcholine- induced relaxation in female diabetic rats // Undersea & hyperbaric medicine, 46 (2019), 5; 635-646 (međunarodna recenzija, članak, znanstveni)


Naslov
Hyperbaric oxygenation affects acetylcholine- induced relaxation in female diabetic rats

Autori
Manojlović, Dragan ; Stupin, Ana ; Mihaljević, Zrinka ; Matić, Anita ; Lenasi, Helena ; Drenjančević, Ines

Kolaboracija
Institute of Physiology, Faculty of Medicine University of Ljubljana, Ljubljana, Slovenia

Izvornik
Undersea & hyperbaric medicine (1066-2936) 46 (2019), 5; 635-646

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
hyperbaric oxygen ; diabetes ; endothelium ; epoxyeicosatrienoic acids ; 20-HETE ; female rats ; nitric oxide ; acetylcholine

Sažetak
We aimed to assess the effects of intermittent hyperbaric oxygenation (HBO2 at 2 bars for 120 minutes a day for four successive days) on acetylcholine-induced vasorelaxation (AChIR) in female Sprague-Dawley (SD) rats (N=80) that were randomized into four groups: healthy controls (CTR) ; diabetic rats (DM) ; and control and diabetic rats that underwent hyperbaric oxygenation (CTR+HBO and DM+HBO), respectively. AChIR was measured in vitro in aortic rings, with/without L-NAME, MS-PPOH, HET0016 or indomethacin. mRNA expression of eNOS, iNOS, COX-1, COX-2, thromboxane A synthase 1 (TBXAS1), CYP4A1, CYP4A3 and CYP2J3 was assessed by qPCR. Systemic oxidative stress and plasma antioxidative capacity were determined with the thiobarbituric acid- reactive substances (TBARS) and the ferric reducing ability of plasma (FRAP) assays, respectively. There was no significant difference in AChIR among experimental groups of rats. In CTR and DM group of rats, AChIR was mediated by NO and EETs pathway, while in the CTR+HBO and DM+HBO groups, NO-pathway prevailed. iNOS expression was upregulated in the DM group compared to CTR, while HBO2 upregulated eNOS in CTR group and TBXAS1 in DM group of rats. In both, CTR and DM group of rats, the sensitivity to ACh in the presence of L-NAME or in the presence of MSPPOH was significantly decreased compared to the response to ACh in the absence or presence of Indomethacin or HET0016. DM and DM+HBO rats had increased TBARS compared to their respective controls. In conclusion, HBO2 presumably alters vasorelaxation in response to ACh from NO-EETs mediated pathways to solely NO-pathway, without affecting oxidative status of DM rats.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
219-2160133-2034 - Djelovanje kisika na vaskularnu funkciju u zdravlju i bolesti (Ines Drenjančević, )
Faculty of Medicine Osijek VIF grant #VIFMEFOS-1

Ustanove
Medicinski fakultet, Osijek,
Fakultet za dentalnu medicinu i zdravstvo, Osijek

Profili:

Avatar Url Ines Drenjančević (autor)

Avatar Url Anita Matić (autor)

Avatar Url Zrinka Mihaljević (autor)

Avatar Url Ana Stupin (autor)

Citiraj ovu publikaciju

(Institute of Physiology, Faculty of Medicine University of Ljubljana, Ljubljana, Slovenia) Manojlović, Dragan; Stupin, Ana; Mihaljević, Zrinka; Matić, Anita; Lenasi, Helena; Drenjančević, Ines
Hyperbaric oxygenation affects acetylcholine- induced relaxation in female diabetic rats // Undersea & hyperbaric medicine, 46 (2019), 5; 635-646 (međunarodna recenzija, članak, znanstveni)
(Institute of Physiology, Faculty of Medicine University of Ljubljana, Ljubljana, Slovenia) (Institute of Physiology, Faculty of Medicine University of Ljubljana, Ljubljana, Slovenia) Manojlović, Dragan, Stupin, A., Mihaljević, Z., Matić, A., Lenasi, H. & Drenjančević, I. (2019) Hyperbaric oxygenation affects acetylcholine- induced relaxation in female diabetic rats. Undersea & hyperbaric medicine, 46 (5), 635-646.
@article{article, year = {2019}, pages = {635-646}, keywords = {hyperbaric oxygen, diabetes, endothelium, epoxyeicosatrienoic acids, 20-HETE, female rats, nitric oxide, acetylcholine}, journal = {Undersea and hyperbaric medicine}, volume = {46}, number = {5}, issn = {1066-2936}, title = {Hyperbaric oxygenation affects acetylcholine- induced relaxation in female diabetic rats}, keyword = {hyperbaric oxygen, diabetes, endothelium, epoxyeicosatrienoic acids, 20-HETE, female rats, nitric oxide, acetylcholine} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE