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Bioactivation of quinolines in a recombinant estrogen receptor transactivation assay is catalyzed by N-methyltransferases (CROSBI ID 262117)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Brinkmann, Markus ; Barz, Bogdan ; Carrière, Danielle ; Velki, Mirna ; Smith, Kilian ; Meyer- Alert, Henriette ; Müller, Yvonne ; Thalmann, Beat ; Bluhm, Kerstin ; Schiwy, Sabrina et al. Bioactivation of quinolines in a recombinant estrogen receptor transactivation assay is catalyzed by N-methyltransferases // Chemical research in toxicology, 32 (2019), 4; 698-707

Podaci o odgovornosti

Brinkmann, Markus ; Barz, Bogdan ; Carrière, Danielle ; Velki, Mirna ; Smith, Kilian ; Meyer- Alert, Henriette ; Müller, Yvonne ; Thalmann, Beat ; Bluhm, Kerstin ; Schiwy, Sabrina ; Hotz, Simone ; Salowsky, Helena ; Tiehm, Andreas ; Hecker, Markus ; Hollert, Henner

engleski

Bioactivation of quinolines in a recombinant estrogen receptor transactivation assay is catalyzed by N-methyltransferases

Hydroxylation of polyaromatic compounds through cytochromes P450 (CYPs) is known to result in potentially estrogenic transformation products. Recently, there has been an increasing awareness of the importance of alternative pathways such as aldehyde oxidases (AOX) or N- methyltransferases (NMT) in bioactivation of small molecules, particularly N-heterocycles. Therefore, this study investigated the biotransformation and activity of methylated quinolines, a class of environmentally relevant N-heterocycles that are no native ligands of the estrogen receptor (ER), in the estrogen- responsive cell line ERα CALUX. We found that this widely-used cell line over-expresses AOXs and NMTs while having low expression of CYP enzymes. Exposure of ERα CALUX cells to quinolines resulted in estrogenic effects, which could be mitigated using an inhibitor of AOX/NMTs. No such mitigation occurred after co- exposure to a CYP1A inhibitor. A number of N- methylated but no hydroxylated transformation products were detected using LC-MS, indicating that biotransformations to estrogenic metabolites were likely catalyzed by NMTs. Compared to the natural ER ligand 17β- estradiol, the products formed during the metabolization of quinolines were weak to moderate agonists of the human ERα. Our findings have potential implications for the risk assessment of these compounds and indicate that care must be taken when using in vitro estrogenicity assays, e.g. ERα CALUX, for the characterization of N-heterocycles or environmental samples that may contain them.

ERα CALUX ; estrogenicity ; hetero-PAHs ; NSO-PAC ; Methylation ; Aldehyde oxidase

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Podaci o izdanju

32 (4)

2019.

698-707

objavljeno

0893-228X

1520-5010

Povezanost rada

Biologija

Indeksiranost