Stability of faecal calprotectin extracts (CROSBI ID 673972)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Vrtarić, Alen ; Božović, Marija ; Nikolac Gabaj, Nora ; Culej, Jelena ; Milevoj Kopčinović, Lara ; Herceg, Ivona
engleski
Stability of faecal calprotectin extracts
BACKGROUND-AIM: The BÜHLMANN CALEX® Cap (BÜHLMANN Laboratories AG, Switzerland) is a single use tube filled with extraction buffer used for extraction of calprotectin (fCal) from human stool samples. According to manufacturer’s specifications, fCal extract is stable 6 days at 2-8 °C. The aim of this study was to verify whether the manufacturer’s criteria are met and test if fCal extracts are stable if stored for a longer period (i.e. for 10 days at 2-8 °C). METHODS: Faecal calprotectin was extracted from N = 10 stool samples according to the manufacturer’s specified protocol. Extracts were then aliquoted in 6 aliquots ; initial fCal was measured on the day of extraction, while the remaining aliquots were stored at 2-8 °C for 1, 3, 5, 7 and 10 days, respectively. Measurements were made on the Abbott Architect c8000 (Abbott Laboratories, Abbott Park, USA) using BÜHLMANN fCAL® turbo reagent (BÜHLMANN Laboratories AG, Switzerland). Bias from the initial fCal concentration was calculated for each individual sample. Mean bias was calculated for each storage period and compared to Instand criteria which is 36%. Statistical analysis was performed using Medcalc (Ostend, Belgium) Friedman ANOVA test. P-value was set at < 0.05. RESULTS: The initial fCal concentration expressed as median (interquartile range) was 198.8 (58.7-731.9) mg/kg. Median concentrations measured in extracts stored for 1, 3, 5, 7 and 10 days were 213.5 (62.4-716.3), 204.5 (54.4-690.3), 207.0 (51.2-682.4), 205.0 (53.7-679.5) and 189.3 (56.2-657.6) mg/kg, respectively. Bias from the initial fCal concentration for extracts stored for 1, 3, 5, 7 and 10 days were -3.52 ; -4.41 ; -0.26 ; -7.03 and -3.41%, respectively. No difference between the initial fCal and fCal measured in extracts stored in the observed time periods was found (P = 0.830). CONCLUSIONS: Faecal calprotectin extract stability met the criteria specified by the manufacturer. We confirmed that fCal extracts might be stored for periods up to 10 days at 2- 8 °C.
faecal calprotectin, extraction, stool
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Podaci o prilogu
eA42-eA42.
2019.
nije evidentirano
objavljeno
10.1515/cclm-2019-0104
Podaci o matičnoj publikaciji
Clinical chemistry and laboratory medicine
Plebani, Mario
Berlin: Walter de Gruyter
1434-6621
1437-4331
Podaci o skupu
5th EFLM Conference on Preanalytical Phase
poster
22.03.2019-23.03.2019
Zagreb, Hrvatska
Povezanost rada
Kliničke medicinske znanosti