Verification of Minicollect Z serum tubes with and without gel (CROSBI ID 673971)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Herceg, Ivona ; Ćelap, Ivana ; Nikolac Gabaj, Nora ; Božović, Marija ; Vrtarić, Alen ; Topić, Anita ; Krtanjek, Sanja
engleski
Verification of Minicollect Z serum tubes with and without gel
BACKGROUND-AIM: Minicollect® Z tubes manufacturer has developed a new tube containing same composition, different shape and better features compared to the old one. Therefore, they need to be verified before introduction into routine work. METHODS: Our study included comparison as follows: 1. qualitative comparison of serum separation ease ; 2. hemolysis degree comparison ; 3. comparison of 14 biochemical parameters (ALT, AMY, AST, direct bilirubin, total bilirubin, Ca, CK, Na, K, Cl, CRP, Glc, enzymatic creatinine, LD) and serum indices (H-hemolysis, I-icteria, L-lipemia). First comparison was done between old and new Minicollect® Z Serum tube (Greiner Bio-One, Kremmsmunster, Austria) (N = 30) and second between new Minicollect® Z Serum and a new Minicollect® Z Serum (Separator-with gel) tube (N = 40). Samples were centrifuged at 12100g/5 min and analyzed on Abbott Architect c8000 (Abbott Laboratories, Abbott Park, Illinois, USA). Sample was considered hemolysed if H index was >0.5. Bland-Altman plots were calculated using Medcalc® v18.10.2 (Ostend, Belgium). Bland- Altman plots derived biases were compared to Westgard’s minimum specification for allowable total error. RESULTS: New tube has an integrated collection scoop and is wider compared to the old one, resulting in easier sampling but lower serum column which makes aliquoting more difficult. However, it’s easier to separate serum in gel tube than from the one without gel. Hemolysed samples proportion didn’t differ between old (7/30) and new (10/30) tubes without gel, P = 0.748, as well between tubes without (9/40) and with gel (16/40), P = 0.148. Proportional bias for AMY (26.3% ; agreement range:-62.8% to 115.3 %) exceeds Westgard’s criteria (21.9%). CONCLUSIONS: Study showed that old tube and gel tube are better for serum separation compared to the new tube and tube without gel. AMY activity is higher in old tubes and possible cause should be tested in further investigations. Finally, gel tube is more appropriate for our routine laboratory work.
Minicollect® tube, new model, verification
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Podaci o prilogu
eA15-eA15.
2019.
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objavljeno
10.1515/cclm-2019-0104
Podaci o matičnoj publikaciji
Clinical chemistry and laboratory medicine
Plabani, Mario
Berlin: Walter de Gruyter
1434-6621
Podaci o skupu
5th EFLM Conference on Preanalytical Phase
poster
22.03.2019-23.03.2019
Zagreb, Hrvatska
Povezanost rada
nije evidentirano