Napredna pretraga

Pregled bibliografske jedinice broj: 990210

Verification of Minicollect Z serum tubes with and without gel

Herceg, Ivona; Ćelap, Ivana; Nikolac Gabaj, Nora; Božović, Marija; Vrtarić, Alen; Topić, Anita; Krtanjek, Sanja
Verification of Minicollect Z serum tubes with and without gel // Clinical Chemistry and Laboratory Medicine / Plabani, Mario (ur.).
Berlin: Walter de Gruyter, 2019. str. eA15-eA15 doi:10.1515/cclm-2019-0104 (poster, međunarodna recenzija, sažetak, stručni)

Verification of Minicollect Z serum tubes with and without gel

Herceg, Ivona ; Ćelap, Ivana ; Nikolac Gabaj, Nora ; Božović, Marija ; Vrtarić, Alen ; Topić, Anita ; Krtanjek, Sanja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Clinical Chemistry and Laboratory Medicine / Plabani, Mario - Berlin : Walter de Gruyter, 2019, EA15-eA15

5th EFLM Conference on Preanalytical Phase

Mjesto i datum
Zagreb, Hrvatska, 22-23.03.2019

Vrsta sudjelovanja

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Minicollect® tube, new model, verification

BACKGROUND-AIM: Minicollect® Z tubes manufacturer has developed a new tube containing same composition, different shape and better features compared to the old one. Therefore, they need to be verified before introduction into routine work. METHODS: Our study included comparison as follows: 1. qualitative comparison of serum separation ease ; 2. hemolysis degree comparison ; 3. comparison of 14 biochemical parameters (ALT, AMY, AST, direct bilirubin, total bilirubin, Ca, CK, Na, K, Cl, CRP, Glc, enzymatic creatinine, LD) and serum indices (H-hemolysis, I-icteria, L-lipemia). First comparison was done between old and new Minicollect® Z Serum tube (Greiner Bio-One, Kremmsmunster, Austria) (N = 30) and second between new Minicollect® Z Serum and a new Minicollect® Z Serum (Separator-with gel) tube (N = 40). Samples were centrifuged at 12100g/5 min and analyzed on Abbott Architect c8000 (Abbott Laboratories, Abbott Park, Illinois, USA). Sample was considered hemolysed if H index was >0.5. Bland-Altman plots were calculated using Medcalc® v18.10.2 (Ostend, Belgium). Bland- Altman plots derived biases were compared to Westgard’s minimum specification for allowable total error. RESULTS: New tube has an integrated collection scoop and is wider compared to the old one, resulting in easier sampling but lower serum column which makes aliquoting more difficult. However, it’s easier to separate serum in gel tube than from the one without gel. Hemolysed samples proportion didn’t differ between old (7/30) and new (10/30) tubes without gel, P = 0.748, as well between tubes without (9/40) and with gel (16/40), P = 0.148. Proportional bias for AMY (26.3% ; agreement range:-62.8% to 115.3 %) exceeds Westgard’s criteria (21.9%). CONCLUSIONS: Study showed that old tube and gel tube are better for serum separation compared to the new tube and tube without gel. AMY activity is higher in old tubes and possible cause should be tested in further investigations. Finally, gel tube is more appropriate for our routine laboratory work.

Izvorni jezik


KBC "Sestre Milosrdnice"

Autor s matičnim brojem:
Nora Nikolac, (266202)

Časopis indeksira:

  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
  • Scopus