engleski

Molecular analysis of HLA-DRB1, - DQA1, -DQB1, -DQCAR, haplotypic associations in Croatian patients with psoriasis

The purpose of the present study was to investigate polymorphism of HLA class II haplotypic associations (HLA-DRB1, -DQA1, -DQB1) and DQCAR alleles in seventy eight Croatian patients with psoriasis. Patients were divided into two groups according to a family history of disease and age of onset: type I (positive family history and early onset) and type II (negative family history and late onset). The difference in frequency of HLA class II haplotypic associations between type I patients and controls was observed for the following combinations: HLA-DRB1*0701, -DQA1*0201, -DQB1*02 (23.6% vs 7.2% ; p<0.001), HLA-DRB1*0701, -DQA1*0201, -DQB1*0303 (8.5% vs 1.3% ; (p=0.0018) and HLA-DRB1*1601, -DQA1*0102, -DQB1*0502 (2.8% vs 9.3% ; p=0.06). The difference between type II psoriasis and controls for association: HLA-DRB1*1501, -DQA1*0102, -DQB1*0602 is not significant (20.0% vs 8.9% ; p=0.06). The significantly higher frequency of DQCAR 113bp and 119bp alleles in patients with type I psoriasis is a result of linkage disequlibrium of these alleles with both HLA-DRB1*0701 haplotypic associations. Analysis of DQCAR alleles in the HLA-DRB1*0701 haplotypic associations in patients with psoriasis vulgaris and matched controls did not reveal any difference in polymorphism of DQCAR alleles. These data suggest that HLA-DRB*0701 haplotypic combinations are associated with type I but not for type II psoriasis in the Croatian population. DQCAR polymorphism is not useful genetic marker to distinguish susceptible HLA class II haplotypic association.

DQCAR; polymorphism; microsatellite; HLA class II genes; psoriasis

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