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Polymethacrylate-based monoliths as stationary phases for separation of biopolymers and immobilization of enzymes (CROSBI ID 261584)

Prilog u časopisu | stručni rad | međunarodna recenzija

Martinović, Tamara ; Josić, Djuro Polymethacrylate-based monoliths as stationary phases for separation of biopolymers and immobilization of enzymes // Electrophoresis, 38 (2017), 22-23; 2821-2826. doi: 10.1002/elps.201700255

Podaci o odgovornosti

Martinović, Tamara ; Josić, Djuro

engleski

Polymethacrylate-based monoliths as stationary phases for separation of biopolymers and immobilization of enzymes

The experiences in the production and application of polymethacrylate-based monolithic supports, since their development almost thirty years ago, are presented. The main driving force for the development of new chromatographic supports was the necessity for the isolation and separation of physiologically active biopolymers and their use for therapeutic purposes. For this sake, a development of a method for fast separation, preventing denaturation and preserving their biological activity was necessary. Development of polysaccharide-based supports, followed by the introduction of polymer-based chromatographic media, is shortly described. This development was followed by the advances in monolithic media that are now used for both large- and small-scale separation of biopolymers and nanoparticles. Finally, a short overview is given about the applications of monoliths for sample displacement chromatography, resulting in isolation of physiologically active biomolecules, such as proteins, protein complexes, and nucleic acid, as well as high-throughput sample preparation for proteomic investigations.

Monolithic stationary phases ; Sample displacement chromatography ; Sample preparation

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Podaci o izdanju

38 (22-23)

2017.

2821-2826

objavljeno

0173-0835

10.1002/elps.201700255

Povezanost rada

Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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