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FTIR spectroscopy of thin films made of human DNA

Investigations of DNA vibrational properties using Fourier transform infrared spectroscopy can be traced back to 1960s. There is a high interest among researchers for this method given the fact that it's nondestructive and suitable for investigation of DNAs vibrational properties which are related to the DNAs structural properties. In most cases, fragments are short sequences of DNA which are extracted from Salomon testes or calves gland timus. In this type of research, there is a little investigation of DNA from biological tissues [1] and even less on human DNA [2]. FTIR spectrum of DNA with the range of wave numbers from 4000 to 400 cm−1 contains about 40 spectral lines which are the result of the DNAs double stranded structure. Range from 1800 to 800 cm−1 is of special interest because in this range we can nd the most vibrations that can be divided into two groups: vibrations of bases and backbone of DNA [3]. In this research, we have used DNA that is extracted from the human placenta of regular pregnancies (archive samples of CERRM). Measurements using infrared spectroscopy were performed on thin lms that were made from DNA solutions. Normalization and baseline correction was performed using program Kinetics which is part of the MatLab software and in program eFTIR. This processed spectra will enable us to assign the certain characteristic vibrational bands so we can compare them with relevant information and nally with the pathological human placenta.

DNA ; spectra ; FTIR ; placenta

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