engleski

Expression of Superoxide Dismutase Enzyme in Stress Condition

Reactive oxygen species (ROS) play a key role in the initiation and progression of many human diseases. Superoxide anion radical (O2•-), arising continuously in the cells, is one of the most important and biologically relevant ROS radicals in living organisms. Superoxide dismutase (SOD) is an enzyme important in the process of cellular responses to stress conditions. Using WST spectrophotometric method, it is investigated the effect of insulin dependent diabetes mellitus (IDDM) on the SOD expression of tissues in aging rats. For this purpose, rat model for IDDM was established by streptozotocin application. The rats were randomly divided into 4 groups of animals: DM-2W (streptozotocin treated and sacrificed after 2 weeks of diabetes), control-2W (control group sacrificed after 2 weeks of the experiment), DM- 1Y (streptozotocin treated and sacrificed after 1 year of diabetes) and control-1Y (control group sacrificed after 1 year of the experiment). Results showed overexpression of SOD enzyme in both of DM-2W and DM-1Y groups compared with control groups of rats. In comparison the expression of the enzyme in both of DM-2W and DM-1Y groups, the stronger overexpression of the enzyme in the first one group was registered. That could be explained by fading of defence system of organism.

SOD ; rat model ; stress conditions ; WST method

nije evidentirano