engleski

Morphometry of freshwater organisms as useful method in ecotoxicology

Ecotoxicological observations of cytological measurements were performed on the freshwater planarian Polycelis felina (Dalyell, 1814) and cnidarian Hydra viridissima (Pallas, 1766) with the aim to determine morphometrical changes of their cell structures. P. felina was exposed to aluminium (80 and 100 mgL-1 for 48 h). H. viridissima was exposed to cinoxacin (1.9x10-3 and 3.8x10-3 mol/L for 72h) and chloramphenicol (2x10-4 mol/L for 24h and 3x10-4 mol/L for 24 and 48h). Both species were exposed to herbicide norflurazon (2x10-6 mol/L) for 3, 24 and 48h. In hydras morphometrical changes were observed in number and area of zymogen and interstitial cells, area of ectoderm and gastroderm, mesoglea thickness, size of ectodermal myoepithelial cells and diameter and area of endosymbiotic algae. In planarians morphometrical changes were observed in the size and number of neoblasts and in the size of the reticular cells, rhabdites and rhabdite layer. The results of morphometric analysis showed changed structures of different cell types, significantly correlating with an increase of toxicant concentration and longer exposure, compared with the control samples. Morphometry proved to be a powerful tool in research of ecotoxicological impact of xenobiotics upon freshwater organisms.

morphometry ; ecotoxicology ; freshwater

ovo je sažetak u zborniku skupa