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Stability of enzyme activities in serum sample – alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and their activated forms (aALT, aAST) (CROSBI ID 672822)

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Brenčić, Tina ; Topić, Anita ; Nikolac, Nora Stability of enzyme activities in serum sample – alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and their activated forms (aALT, aAST) // Clinical chemistry and laboratory medicine. 2017. str. A31-A31

Podaci o odgovornosti

Brenčić, Tina ; Topić, Anita ; Nikolac, Nora

engleski

Stability of enzyme activities in serum sample – alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and their activated forms (aALT, aAST)

Background: Activated alanine aminotransferase (aALT) and activated aspartate aminotransferase (aAST) are IFCC recommended methods with pyridoxal-6-phosphate as a reaction activator, unlike ALT and AST. Manufacturer declares the stability of serum sample for enzyme activity at room temperature and 4°C without specification of sample type (primary tube or aliquot). The aim of this study was to evaluate stability of aALT, ALT, aAST and AST in primary tube and aliquot at both temperatures. Materials and methods: Study was carried out in University Department of Chemistry, Medical School University Hospital Sestre Milosrdnice (Zagreb, Croatia) from May to June 2016. Manufacturer’s declared stabilities are: ALT, aALT: 3 days ; AST, aAST: 4 days at room temperature and ALT, aALT ; AST, aAST- 7 days at 4°C. Four serum samples were used for the study: aliquoted serum sample stored at room temperature and at 2-8°C and serum sample in primary tube at both temperatures. Enzyme activities were measured daily during 10 days in duplicate on Architect c8000 analyser (Abbott, Illinois, USA) using original reagent kits. Mean value between two measurements and bias(%) for each sample regarding the initial activity was calculated. Acceptance criteria was defined as external quality control criteria (ALT, aALT- 12% ; AST, aAST- 15%). Results: Enzyme stability for ALT and aAST confirmed manufacturer’s declarations. aALT activity didn’t meet acceptance criteria: it was stable 1 day (aliquoted sample, day 2 bias = 33.3%) and 4 days (primary tube ; day 5 bias = -12.7%) at room temperature and 6 days (aliquoted sample, day 7 bias -15.6% ) at 2- 8°C, while the activity in primary tube at 2- 8°C wasn’t changed during the tested period. In primary tube sample at room temperature, AST activity was stable 3 days (day 4 bias = 18.3%). Conclusion: ALT and aAST are more stable enzymes than aALT and AST. Enzymes are stable longer when samples are stored at 2-8°C.

preanalytical phase ; sample stability ; AST ; ALT

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Podaci o prilogu

A31-A31.

2017.

nije evidentirano

objavljeno

Podaci o matičnoj publikaciji

Clinical chemistry and laboratory medicine

1434-6621

1437-4331

Podaci o skupu

4th EFLM-BD European Conference on Preanalytical Phase – Improving Quality of Preanalytical Phase through inovation

poster

24.03.2017-25.03.2017

Amsterdam, Nizozemska

Povezanost rada

Kliničke medicinske znanosti

Indeksiranost