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Internal quality assurance of HIL indices on Roche Cobas c702 (CROSBI ID 260453)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Lippi, Giuseppe ; Cadamuro, Janne ; Danese, Elisa ; Gelati, Matteo ; Montagnana, Martina ; von Meyer, Alexander ; Salvagno, Gian Luca ; Šimundić, Ana-Maria Internal quality assurance of HIL indices on Roche Cobas c702 // PLoS One, 13 (2018), 7; 0200088, 11. doi: 10.1371/journal.pone.0200088

Podaci o odgovornosti

Lippi, Giuseppe ; Cadamuro, Janne ; Danese, Elisa ; Gelati, Matteo ; Montagnana, Martina ; von Meyer, Alexander ; Salvagno, Gian Luca ; Šimundić, Ana-Maria

engleski

Internal quality assurance of HIL indices on Roche Cobas c702

Automatic assessment of hemoglobin (H), lipaemia (L) and icterus (I) in serum or plasma (HIL indices) is the mainstay for evaluating sample quality. We planned this study to verify whether in-house prepared internal quality control (IQC) materials may be suitable for quality assurance of HIL indices. Pools containing different values of each of the three HIL indices were prepared from routine plasma samples, divided in aliquots and frozen at -20°C. Stability of frozen materials was assessed by thawing one aliquot of each pool after different days of freezing (1, 4, 8, 15, 22 and 29), and by measuring HIL indices on baseline fresh samples and frozen-thawed aliquots with Roche Cobas c702. Five fresh liquid IQCs materials were also measured at the same time points. Intra-assay and inter-assay imprecision of HIL indices calculated with commercial IQC materials ranged between 1.1–2.0% and 1.6–3.3%, respectively. When target values of HIL indices were calculated using frozen-thawed aliquots, the inter-assay imprecision of in-house prepared materials was optimal, even better than that of commercial liquid IQCs (H-index, 0.8% versus 1.6% ; L-index, 2.2% versus 2.5% ; I-index, 0.8% versus 3.3%). In conclusion, in-house prepared IQC materials are cost-effective alternatives to commercial liquid IQCs for HIL quality assurance.

HIL

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

13 (7)

2018.

0200088

11

objavljeno

1932-6203

10.1371/journal.pone.0200088

Povezanost rada

Kliničke medicinske znanosti

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